SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGIC2
Systematic nameYDR309C
DescriptionGTPase-interacting component 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1  CDC34RINGSCFGRR1   
2   RINGSCFGRR1   
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex."
Jaquenoud M, Gulli MP, Peter K, Peter M.
EMBO J. 17(18):5360-73 (1998) [9736614]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"F-box protein Grr1 interacts with phosphorylated targets via the cationic surface of its leucine-rich repeat."
Hsiung YG, Chang HC, Pellequer JL, La Valle R, Lanker S, Wittenberg C.
Mol Cell Biol. 21(7):2506-20 (2001) [11259599]
[3]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionQ06648
SGD YDR309C
Gene Ontology (GO)
Cellular componentGO:0030478actin capIDA:SGD.
Cellular componentGO:0005934cellular bud tipIDA:SGD.
Cellular componentGO:0000131incipient cellular bud siteIDA:SGD.
Cellular componentGO:0043332mating projection tipIDA:SGD.
Molecular functionGO:0005083small GTPase regulator activityTAS:SGD.
Biological processGO:0007120axial cellular bud site selectionIMP:SGD.
Biological processGO:0007096regulation of exit from mitosisIMP:SGD.
Biological processGO:0007266Rho protein signal transductionIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
99Phosphoserine.
254Phosphoserine.
258Phosphoserine.
337Phosphoserine.
345Phosphoserine.
367Phosphoserine.

Sequence Information
Sequence length383
Molecular weight42852

	---------+----------+----------+----------+----------+
MTSASITNTG NETMNLPQMR SIWLDEDEEA EKLYGLQAQQ FMGSDDEENL   50
GITFINSDKP VLSNKKNIEL PPLSPNSHPS CHHRRSNSNS AKSKESSSSS   100
SSANKTNHKK VFLKLNLLKK KLLGAQPDIR GKGISTPFDF QHISHADTRN   150
GFQDEQLQEP SSLSTEIKDD YTSSSSKRDS KSLNKAFVTE RIPANRESKL   200
ISRSHENKTS RLSVARSISV TSSNYSKNTQ GNNHSINGRV VSTSTMATSI   250
FEYSPNASPK QFKNKSHALG HRYTNSTDSS ESSLDFLKNY NFPTLLEDKP   300
ILDFLPRSQR SSAYRSLLET PNSNKDSAKA FFPSRQSPLP KRRNSIATPS   350
PQSKFSYSDS PVNHRKSFDD VLYSFNQLEP LQT                     383