SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGFA1
Systematic nameYKL104C
DescriptionGlucosamine--fructose-6-phosphate aminotransferase [isomerizing]

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP14742
SGD YKL104C
Gene Ontology (GO)
Molecular functionGO:0004360glutamine-fructose-6-phosphate transaminase...IDA:SGD.
Biological processGO:0006038cell wall chitin biosynthetic processIGI:SGD.
EC number 2.6.1.16

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
199Phosphoserine.
332Phosphoserine.

Sequence Information
Sequence length717
Molecular weight80047

	---------+----------+----------+----------+----------+
MCGIFGYCNY LVERSRGEII DTLVDGLQRL EYRGYDSTGI AIDGDEADST   50
FIYKQIGKVS ALKEEITKQN PNRDVTFVSH CGIAHTRWAT HGRPEQVNCH   100
PQRSDPEDQF VVVHNGIITN FRELKTLLIN KGYKFESDTD TECIAKLYLH   150
LYNTNLQNGH DLDFHELTKL VLLELEGSYG LLCKSCHYPN EVIATRKGSP   200
LLIGVKSEKK LKVDFVDVEF PEENAGQPEI PLKSNNKSFG LGPKKAREFE   250
AGSQNANLLP IAANEFNLRH SQSRAFLSED GSPTPVEFFV SSDAASVVKH   300
TKKVLFLEDD DLAHIYDGEL HIHRSRREVG ASMTRSIQTL EMELAQIMKG   350
PYDHFMQKEI YEQPESTFNT MRGRIDYENN KVILGGLKAW LPVVRRARRL   400
IMIACGTSYH SCLATRAIFE ELSDIPVSVE LASDFLDRKC PVFRDDVCVF   450
VSQSGETADT MLALNYCLER GALTVGIVNS VGSSISRVTH CGVHINAGPE   500
IGVASTKAYT SQYIALVMFA LSLSDDRVSK IDRRIEIIQG LKLIPGQIKQ   550
VLKLEPRIKK LCATELKDQK SLLLLGRGYQ FAAALEGALK IKEISYMHSE   600
GVLAGELKHG VLALVDENLP IIAFGTRDSL FPKVVSSIEQ VTARKGHPII   650
ICNENDEVWA QKSKSIDLQT LEVPQTVDCL QGLINIIPLQ LMSYWLAVNK   700
GIDVDFPRNL AKSVTVE                                       717