SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGEA1
Systematic nameYJR031C
DescriptionARF guanine-nucleotide exchange factor 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP47102
SGD YJR031C
Gene Ontology (GO)
Cellular componentGO:0000137Golgi cis cisternaIDA:SGD.
Cellular componentGO:0005798Golgi-associated vesicleIPI:SGD.
Molecular functionGO:0005086ARF guanyl-nucleotide exchange factor activityTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0030036actin cytoskeleton organization and biogenesisIGI:SGD.
Biological processGO:0006888ER to Golgi vesicle-mediated transportIMP:SGD.
Biological processGO:0006891intra-Golgi vesicle-mediated transportIPI:SGD.
Biological processGO:0006890retrograde vesicle-mediated transport, Golg...IGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1408
Molecular weight159437

	---------+----------+----------+----------+----------+
MHDVPMETVL AVNPATMIVK ECINLCSAMN KQSRDKSQTS VAALLGGGSD   50
IFLSQSDSFV DSFHNLPTSS YHDPLISGLV QLRLKINDLK GLDSLNALEL   100
LKPFLEIVSA SSVSGYTTSL ALDSLQKVFT LKIINKTFND IQIAVRETVV   150
ALTHCRFEAS KQISDDSVLL KVVTLLRDII TSSFGDYLSD TIIYDVLQTT   200
LSLACNTQRS EVLRKTAEVT IAGITVKLFT KLKLLDPPTK TEKYINDESY   250
TDNNLKDDII GTTTSDNDLS STDDDSAVAD DNKNEKPVQQ VIREQENDEE   300
TAEKAENVEP NYGITVIKDY LGLLLSLVMP ENRMKHTTSA MKLSLQLINA   350
AIEISGDKFP LYPRLFSLIS DPIFKSVLFI IQSSTQYSLL QATLQLFTSL   400
VVILGDYLPM QIELTLRRIF EILEDTTISG DVSKQKPPAI RELIIEQLSI   450
LWIHSPAFFL QLFVNFDCNL DRSDLSIDFI KELTKFSLPA AAVNTSNNIP   500
PICLEGVLSL IENIYNDLQR FDRAEFVKNQ KEIDILKQRD RKTEFILCVE   550
TFNEKAKKGI QMLIEKGFID SDSNRDIASF LFLNNGRLNK KTIGLLLCDP   600
KKTSLLKEFI DLFDFKGLRV DEAIRILLTK FRLPGESQQI ERIVEAFSSK   650
YSADQSNDKV ELEDKKAGKN GSESMTEDDI IHVQPDADSV FVLSYSIIML   700
NTDSHNPQVK DHMTFDDYSN NLRGCYNGKD FPRWYLHKIY TSIKVKEIVM   750
PEEHHGNERW FEDAWNNLIS STSVMTEMQR DFTNPISKLA QIDILQYEKA   800
IFSNVRDIIL KTLFKIFTVA SSDQISLRIL DAISKCTFIN YYFSFDQSYN   850
DTVLHLGEMT TLAQSSAKAV ELDVDSIPLV EIFVEDTGSK ISVSNQSIRL   900
GQNFKAQLCT VLYFQIIKEI SDPSIVSTRL WNQIVQLILK LFENLLMEPN   950
LPFFTNFHSL LKLPELPLPD PDISIRKAKM SRSLLSTFAS YLKGDEEPSE   1000
EDIDFSIKAF ECVKASHPLS SVFENNQLVS PKMIETLLSS LVIEKTSENS   1050
PYFEQELLFL LEISIILISE ASYGQEFGAL IADHMINISN LDGLSKEAIA   1100
RLASYKMFLV SRFDNPRDIL SDLIEHDFLV KNEIFNTKYY ESEWGKQVIN   1150
DLFTHLNDVK YNERALKNVK FWNFLRILIS AKDRQFAVYT FLEKYIQNGD   1200
IFVDDGNFMN ILSLLDEMSC AGAVGTKWEQ NYENSVEDGC EAPESNPYRS   1250
IIDLSSRSIN ITADLLSTVG RSNSALNKNE IIAAIQGLAH QCLNPCDELG   1300
MQALQALENI LLSRASQLRT EKVAVDNLLE TGLLPIFELD EIQDVKMKRI   1350
TSILSVLSKI FLGQLVEGVT SNETFLRVLN VFNKYVDDPT VERQLQELII   1400
SKREIEKE                                                 1408