SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGDH3
Systematic nameYAL062W
DescriptionNADP-specific glutamate dehydrogenase 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP39708
SGD YAL062W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0005625soluble fractionIDA:SGD.
Molecular functionGO:0004352glutamate dehydrogenase activityIMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006537glutamate biosynthetic processIGI:SGD.
EC number 1.4.1.4

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length457
Molecular weight49627

	---------+----------+----------+----------+----------+
MTSEPEFQQA YDEIVSSVED SKIFEKFPQY KKVLPIVSVP ERIIQFRVTW   50
ENDNGEQEVA QGYRVQFNSA KGPYKGGLRF HPSVNLSILK FLGFEQIFKN   100
ALTGLDMGGG KGGLCVDLKG KSDNEIRRIC YAFMRELSRH IGKDTDVPAG   150
DIGVGGREIG YLFGAYRSYK NSWEGVLTGK GLNWGGSLIR PEATGFGLVY   200
YTQAMIDYAT NGKESFEGKR VTISGSGNVA QYAALKVIEL GGIVVSLSDS   250
KGCIISETGI TSEQIHDIAS AKIRFKSLEE IVDEYSTFSE SKMKYVAGAR   300
PWTHVSNVDI ALPCATQNEV SGDEAKALVA SGVKFVAEGA NMGSTPEAIS   350
VFETARSTAT NAKDAVWFGP PKAANLGGVA VSGLEMAQNS QKVTWTAERV   400
DQELKKIMIN CFNDCIQAAQ EYSTEKNTNT LPSLVKGANI ASFVMVADAM   450
LDQGDVF                                                  457