SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGDH1
Systematic nameYOR375C
AliasURE1
DescriptionNADP-specific glutamate dehydrogenase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K325;K371        
2         
3 MULTI       
4        Proteasomal Degradation
5         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[5]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP07262
SGD YOR375C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0004354glutamate dehydrogenase (NADP+) activityIDA:SGD.
Biological processGO:0006537glutamate biosynthetic processIGI:SGD.
EC number 1.4.1.4

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length454
Molecular weight49570

	---------+----------+----------+----------+----------+
MSEPEFQQAY EEVVSSLEDS TLFEQHPEYR KVLPIVSVPE RIIQFRVTWE   50
NDKGEQEVAQ GYRVQYNSAK GPYKGGLRFH PSVNLSILKF LGFEQIFKNS   100
LTGLDMGGGK GGLCVDLKGR SNNEIRRICY AFMRELSRHI GQDTDVPAGD   150
IGVGGREIGY LFGAYRSYKN SWEGVLTGKG LNWGGSLIRP EATGYGLVYY   200
TQAMIDYATN GKESFEGKRV TISGSGNVAQ YAALKVIELG GTVVSLSDSK   250
GCIISETGIT SEQVADISSA KVNFKSLEQI VNEYSTFSEN KVQYIAGARP   300
WTHVQKVDIA LPCATQNEVS GEEAKALVAQ GVKFIAEGSN MGSTPEAIAV   350
FETARSTATG PSEAVWYGPP KAANLGGVAV SGLEMAQNSQ RITWTSERVD   400
QELKRIMINC FNECIDYAKK YTKDGKVLPS LVKGANIASF IKVSDAMFDQ   450
GDVF                                                     454

	* Ubiquitinated lysine is denoted as red color.