SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGDA1
Systematic nameYEL042W
DescriptionGuanosine-diphosphatase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32621
SGD YEL042W
Gene Ontology (GO)
Cellular componentGO:0005794Golgi apparatusTAS:SGD.
Molecular functionGO:0004382guanosine-diphosphatase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0045134uridine-diphosphatase activityIDA:SGD.
Biological processGO:0006486protein amino acid glycosylationTAS:SGD.
EC number 3.6.1.42

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length518
Molecular weight56822

	---------+----------+----------+----------+----------+
MAPIFRNYRF AIGAFAVIML ILLIKTSSIG PPSIARTVTP NASIPKTPED   50
ISILPVNDEP GYLQDSKTEQ NYPELADAVK SQTSQTCSEE HKYVIMIDAG   100
STGSRVHIYK FDVCTSPPTL LDEKFDMLEP GLSSFDTDSV GAANSLDPLL   150
KVAMNYVPIK ARSCTPVAVK ATAGLRLLGD AKSSKILSAV RDHLEKDYPF   200
PVVEGDGVSI MGGDEEGVFA WITTNYLLGN IGANGPKLPT AAVFDLGGGS   250
TQIVFEPTFP INEKMVDGEH KFDLKFGDEN YTLYQFSHLG YGLKEGRNKV   300
NSVLVENALK DGKILKGDNT KTHQLSSPCL PPKVNATNEK VTLESKETYT   350
IDFIGPDEPS GAQCRFLTDE ILNKDAQCQS PPCSFNGVHQ PSLVRTFKES   400
NDIYIFSYFY DRTRPLGMPL SFTLNELNDL ARIVCKGEET WNSVFSGIAG   450
SLDELESDSH FCLDLSFQVS LLHTGYDIPL QRELRTGKKI ANKEIGWCLG   500
ASLPLLKADN WKCKIQSA                                      518