SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGCN4
Systematic nameYEL009C
AliasAAS3, ARG9
DescriptionGeneral control protein GCN4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1  RAD6, CDC34     Proteasomal Degradation
2  CDC34RINGSCFCDC4   
3 MULTI    RPN10 Proteasomal Degradation
4 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Regulated degradation of the transcription factor Gcn4."
Kornitzer D, Raboy B, Kulka RG, Fink GR.
EMBO J. 13(24):6021-30 (1994) [7813440]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Degradation of the transcription factor Gcn4 requires the kinase Pho85 and the SCF(CDC4) ubiquitin-ligase complex."
Meimoun A, Holtzman T, Weissman Z, McBride HJ, Stillman DJ, Fink GR, Kornitzer D.
Mol Biol Cell. 11(3):915-27 (2000) [10712509]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP03069
SGD YEL009C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIPI:SGD.
Molecular functionGO:0003700transcription factor activityIDA:SGD.
Biological processGO:0008652amino acid biosynthetic processTAS:SGD.
Biological processGO:0006990unfolded protein response, positive regulat...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
165Phosphothreonine, by PHO85.

Sequence Information
Sequence length281
Molecular weight31310

	---------+----------+----------+----------+----------+
MSEYQPSLFA LNPMGFSPLD GSKSTNENVS ASTSTAKPMV GQLIFDKFIK   50
TEEDPIIKQD TPSNLDFDFA LPQTATAPDA KTVLPIPELD DAVVESFFSS   100
STDSTPMFEY ENLEDNSKEW TSLFDNDIPV TTDDVSLADK AIESTEEVSL   150
VPSNLEVSTT SFLPTPVLED AKLTQTRKVK KPNSVVKKSH HVGKDDESRL   200
DHLGVVAYNR KQRSIPLSPI VPESSDPAAL KRARNTEAAR RSRARKLQRM   250
KQLEDKVEEL LSKNYHLENE VARLKKLVGE R                       281