SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameGCD11
Systematic nameYER025W
AliasTIF213
DescriptionEukaryotic translation initiation factor 2 subunit gamma

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP32481
SGD YER025W
Gene Ontology (GO)
Cellular componentGO:0005850eukaryotic translation initiation factor 2 ...IMP:SGD.
Cellular componentGO:0043614multi-eIF complexIDA:SGD.
Cellular componentGO:0005840ribosomeTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003743translation initiation factor activityTAS:SGD.
Biological processGO:0006413translational initiationTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
258Phosphoserine.

Sequence Information
Sequence length527
Molecular weight57866

	---------+----------+----------+----------+----------+
MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA   50
FTGLEEDGET EEEKRKREFE EGGGLPEQPL NPDFSKLNPL SAEIINRQAT   100
INIGTIGHVA HGKSTVVRAI SGVQTVRFKD ELERNITIKL GYANAKIYKC   150
QEPTCPEPDC YRSFKSDKEI SPKCQRPGCP GRYKLVRHVS FVDCPGHDIL   200
MSTMLSGAAV MDAALLLIAG NESCPQPQTS EHLAAIEIMK LKHVIILQNK   250
VDLMREESAL EHQKSILKFI RGTIADGAPI VPISAQLKYN IDAVNEFIVK   300
TIPVPPRDFM ISPRLIVIRS FDVNKPGAEI EDLKGGVAGG SILNGVFKLG   350
DEIEIRPGIV TKDDKGKIQC KPIFSNIVSL FAEQNDLKFA VPGGLIGVGT   400
KVDPTLCRAD RLVGQVVGAK GHLPNIYTDI EINYFLLRRL LGVKTDGQKQ   450
AKVRKLEPNE VLMVNIGSTA TGARVVAVKA DMARLQLTSP ACTEINEKIA   500
LSRRIEKHWR LIGWATIKKG TTLEPIA                            527