SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGAT2
Systematic nameYMR136W
DescriptionProtein GAT2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP40209
SGD YMR136W
Gene Ontology (GO)
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length560
Molecular weight63139

	---------+----------+----------+----------+----------+
MQAPNIYPFS QTQPQALPGF TYGPPQLVFD HSAPRVDPLH STVTINSPLP   50
LQHYNGPNAH INSANNNYAY YYHHPNNNDN NNHSNNTIKN NNINSVLPAV   100
NIQISNNSHY RNTHQIPSAP QRLVSIIPDP HMPPNISHFQ LNNIHPQMHA   150
PVATDIHFQQ VPVYNKTNNG IGTDNINNDK PVNSNQNEVL DNIDERSCHE   200
INRVVSFSKH FENNELTTTA NDLNIQSTMD ELAKLKSLSN STHFRQSIAT   250
QNFHSLQNHI TTIENRLASL LTDRQQEQQQ LKQQESEKES SSPFSNKIKL   300
PSLQELTDSI STQHLPTFYD NKRHASDTDL KSSTLHGPLY HRHAFLSTSS   350
SSPSPTAGSA PLQKLQVPRQ DDPNDKKMNI SSSPFNSITY IPNTTLSPMV   400
QTQLKNLTTS NLNTKKKNNR GRPRAIQRQP TLTTSSHFIN NSNPGAAAVS   450
TTTPAANSDE KNPNAKKIIE FCFHCGETET PEWRKGPYGT RTLCNACGLF   500
YRKVTKKFGS KSSNLLLRYR RSIDLANDRR IPDFITIPNR FIHDMDNDQT   550
LDSEYNTILQ                                                560