SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameGAS5
Systematic nameYOL030W
DescriptionGlycolipid-anchored surface protein 5

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ08193
SGD YOL030W
Gene Ontology (GO)
Cellular componentGO:0009277chitin- and beta-glucan-containing cell wallIDA:SGD.
Molecular functionGO:00421241,3-beta-glucanosyltransferase activityIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length484
Molecular weight51870

	---------+----------+----------+----------+----------+
MLLRSLTSAF VLSAGLAQAA SSSNSSTPSI EIKGNAFFNS ESGERFYIRG   50
VDYQPGGSSN LTDPLADASV CDRDVPVLKD LGINTVRVYT VDNSQDHSHC   100
MKLLQENGIY LILDVNTPTS AISRYDPACS YNADYLQNVF ATIDTFADYD   150
NVLGFFAGNE VINSVNTTNT ATYVKAVVRD MKKYIKARKY RQIPVGYSAA   200
DIVANRQLAA EYFNCGDEAD ARIDMFGVND YSWCGESSFV VSGYSTKMKL   250
YQDYSVPVFL SEFGCNQVKS SRPFTEIEAI YSTQMSSVFS GGLVYEYSNE   300
TNNYGLVQID GDKVTKLTDF ENLKNEYSKV SNPEGNGGYS TSNNYSTCPD   350
YEKGVWEANN TLPAMPSAAS AYFTSGAGSP MGTGIATQQS CDAKDDDDEE   400
DDDTSSSSSS SSSSSSSASS SSESSSSTSK ASSSSPSASE TSLLKSAASA   450
TSSSQSSSKS KGAAGIIEIP LIFRALAELY NLVL                    484