SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameGAP1
Systematic nameYKR039W
DescriptionGeneral amino-acid permease GAP1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5  Vacuolar Degradation
2K9;K16MULTI(K63-linked) HECTNARSP5  Vacuolar Degradation
3K76        
4K76;K87;K91  HECTNARSP5  Vacuolar Degradation
5 MULTI(K63-linked)     DOA4 
6 MULTI    RPN10 Proteasomal Degradation
7         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Nitrogen-regulated ubiquitination of the Gap1 permease of Saccharomyces cerevisiae."
Springael JY, Andre B.
Mol Biol Cell. 9(6):1253-63 (1998) [9614172]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Ubiquitin is required for sorting to the vacuole of the yeast general amino acid permease, Gap1."
Soetens O, De Craene JO, Andre B.
J Biol Chem. 276(47):43949-57 (2001) [11500494]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
purification (6xHis-Myc-ubiquitin), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]

"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]
[4]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Evidence for coupled biogenesis of yeast Gap1 permease and sphingolipids: essential role in transport activity and normal control by ubiquitination."
Lauwers E, Grossmann G, Andr? B.
Mol Biol Cell. 18(8):3068-80 (2007) [17553927]
[5]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"NH4+-induced down-regulation of the Saccharomyces cerevisiae Gap1p permease involves its ubiquitination with lysine-63-linked chains."
Springael JY, Galan JM, Haguenauer-Tsapis R, Andre B.
J Cell Sci. 112(Pt 9):1375-83 (1999) [10194416]
[6]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[7]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP19145
SGD YKR039W
Gene Ontology (GO)
Cellular componentGO:0030134ER to Golgi transport vesicleIDA:SGD.
Cellular componentGO:0005887integral to plasma membraneIDA:SGD.
Cellular componentGO:0000328lumen of vacuole with cell cycle-correlated...IDA:SGD.
Cellular componentGO:0005771multivesicular bodyIMP:SGD.
Molecular functionGO:0015359amino acid permease activityIMP:SGD.
Molecular functionGO:0005296L-proline permease activityIGI:SGD.
Biological processGO:0006865amino acid transportIMP:SGD.
Biological processGO:0015846polyamine transportIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length602
Molecular weight65656

	---------+----------+----------+----------+----------+
MSNTSSYEKN NPDNLKHNGI TIDSEFLTQE PITIPSNGSA VSIDETGSGS   50
KWQDFKDSFK RVKPIEVDPN LSEAEKVAII TAQTPLKHHL KNRHLQMIAI   100
GGAIGTGLLV GSGTALRTGG PASLLIGWGS TGTMIYAMVM ALGELAVIFP   150
ISGGFTTYAT RFIDESFGYA NNFNYMLQWL VVLPLEIVSA SITVNFWGTD   200
PKYRDGFVAL FWLAIVIINM FGVKGYGEAE FVFSFIKVIT VVGFIILGII   250
LNCGGGPTGG YIGGKYWHDP GAFAGDTPGA KFKGVCSVFV TAAFSFAGSE   300
LVGLAASESV EPRKSVPKAA KQVFWRITLF YILSLLMIGL LVPYNDKSLI   350
GASSVDAAAS PFVIAIKTHG IKGLPSVVNV VILIAVLSVG NSAIYACSRT   400
MVALAEQRFL PEIFSYVDRK GRPLVGIAVT SAFGLIAFVA ASKKEGEVFN   450
WLLALSGLSS LFTWGGICIC HIRFRKALAA QGRGLDELSF KSPTGVWGSY   500
WGLFMVIIMF IAQFYVAVFP VGDSPSAEGF FEAYLSFPLV MVMYIGHKIY   550
KRNWKLFIPA EKMDIDTGRR EVDLDLLKQE IAEEKAIMAT KPRWYRIWNF   600
WC                                                       602

	* Ubiquitinated lysine is denoted as red color.