SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameFUR4
Systematic nameYBR021W
DescriptionUracil permease

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5  Vacuolar Degradation
2 MONO, MULTI(K63-linked)       
3K38;K41        
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Ubiquitination mediated by the Npi1p/Rsp5p ubiquitin-protein ligase is required for endocytosis of the yeast uracil permease."
Galan JM, Moreau V, Andre B, Volland C, Haguenauer-Tsapis R.
J Biol Chem. 271(18):10946-52 (1996) [8631913]
[2]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein."
Galan JM, Haguenauer-Tsapis R.
EMBO J. 16(19):5847-54 (1997) [9312043]
[3]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Casein kinase I-dependent phosphorylation within a PEST sequence and ubiquitination at nearby lysines signal endocytosis of yeast uracil permease."
Marchal C, Haguenauer-Tsapis R, Urban-Grimal D.
J Biol Chem. 275(31):23608-14 (2000) [10811641]

Cross References
Swiss-Prot accessionP05316
SGD YBR021W
Gene Ontology (GO)
Cellular componentGO:0045121lipid raftIDA:SGD.
Molecular functionGO:0015505uracil permease activityIDA:SGD.
Biological processGO:0015857uracil transportIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length633
Molecular weight71736

	---------+----------+----------+----------+----------+
MPDNLSLHLS GSSKRLNSRQ LMESSNETFA PNNVDLEKEY KSSQSNITTE   50
VYEASSFEEK VSSEKPQYSS FWKKIYYEYV VVDKSILGVS ILDSFMYNQD   100
LKPVEKERRV WSWYNYCYFW LAECFNINTW QIAATGLQLG LNWWQCWITI   150
WIGYGFVGAF VVLASRVGSA YHLSFPISSR ASFGIFFSLW PVINRVVMAI   200
VWYSVQAYIA ATPVSLMLKS IFGKDLQDKI PDHFGSPNAT TYEFMCFFIF   250
WAASLPFLLV PPHKIRHLFT VKAVLVPFAS FGFLIWAIRR AHGRIALGSL   300
TDVQPHGSAF SWAFLRSLMG CMANFSTMVI NAPDFSRFSK NPNSALWSQL   350
VCIPFLFSIT CLIGILVTAA GYEIYGINYW SPLDVLEKFL QTTYNKGTRA   400
GVFLISFVFA VAQLGTNISA NSLSCGTDMS AIFPKFINIK RGSLFCAAMA   450
LCICPWNLMA TSSKFTMALS AYAIFLSSIA GVVCSDYFVV RRGYIKLTHI   500
YSHQKGSFYM YGNRFGINWR ALAAYLCGVA PCLPGFIAEV GAPAIKVSDG   550
AMKLYYLSYW VGYGLSFSSY TALCYFFPVP GCPVNNIIKD KGWFQRWANV   600
DDFEEEWKDT IERDDLVDDN ISVYEHEHEK TFI                     633

	* Ubiquitinated lysine is denoted as red color.