SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameFUN12
Systematic nameYAL035W
DescriptionEukaryotic translation initiation factor 5B

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP39730
SGD YAL035W
Gene Ontology (GO)
Cellular componentGO:0005843cytosolic small ribosomal subunit (sensu Eu...IDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0003924GTPase activityIMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003743translation initiation factor activityIDA:SGD.
Biological processGO:0006413translational initiationIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
381Phosphoserine.
386Phosphothreonine.
390Phosphothreonine.

Sequence Information
Sequence length1002
Molecular weight112268

	---------+----------+----------+----------+----------+
MAKKSKKNQQ NYWDEEFEED AAQNEEISAT PTPNPESSAG ADDTSREASA   50
SAEGAEAIEG DFMSTLKQSK KKQEKKVIEE KKDGKPILKS KKEKEKEKKE   100
KEKQKKKEQA ARKKAQQQAQ KEKNKELNKQ NVEKAAAEKA AAEKSQKSKG   150
ESDKPSASAK KPAKKVPAGL AALRRQLELK KQLEEQEKLE REEEERLEKE   200
EEERLANEEK MKEEAKAAKK EKEKAKREKR KAEGKLLTRK QKEEKKLLER   250
RRAALLSSGN VKVAGLAKKD GEENKPKKVV YSKKKKRTTQ ENASEAIKSD   300
SKKDSEVVPD DELKESEDVL IDDWENLALG DDDEEGTNEE TQESTASHEN   350
EDQNQGEEEE EGEEEEEEEE ERAHVHEVAK STPAATPAAT PTPSSASPNK   400
KDLRSPICCI LGHVDTGKTK LLDKIRQTNV QGGEAGGITQ QIGATYFPID   450
AIKAKTKVMA EYEKQTFDVP GLLVIDTPGH ESFSNLRSRG SSLCNIAILV   500
IDIMHGLEQQ TIESIKLLRD RKAPFVVALN KIDRLYDWKA IPNNSFRDSF   550
AKQSRAVQEE FQSRYSKIQL ELAEQGLNSE LYFQNKNMSK YVSIVPTSAV   600
TGEGVPDLLW LLLELTQKRM SKQLMYLSHV EATILEVKVV EGFGTTIDVI   650
LSNGYLREGD RIVLCGMNGP IVTNIRALLT PQPLRELRLK SEYVHHKEVK   700
AALGVKIAAN DLEKAVSGSR LLVVGPEDDE DELMDDVMDD LTGLLDSVDT   750
TGKGVVVQAS TLGSLEALLD FLKDMKIPVM SIGLGPVYKR DVMKASTMLE   800
KAPEYAVMLC FDVKVDKEAE QYAEQEGIKI FNADVIYHLF DSFTAYQEKL   850
LEERRKDFLD YAIFPCVLQT LQIINKRGPM IIGVDVLEGT LRVGTPICAV   900
KTDPTTKERQ TLILGKVISL EINHQPVQEV KKGQTAAGVA VRLEDPSGQQ   950
PIWGRHVDEN DTLYSLVSRR SIDTLKDKAF RDQVARSDWL LLKKLKVVFG   1000
IE                                                       1002