SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameFSH1
Systematic nameYHR049W
DescriptionFamily of serine hydrolases 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38777
SGD YHR049W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0017171serine hydrolase activityIDA:SGD.
EC number 3.1.-.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length243
Molecular weight27339

	---------+----------+----------+----------+----------+
MTVQIPKLLF LHGFLQNGKV FSEKSSGIRK LLKKANVQCD YIDAPVLLEK   50
KDLPFEMDDE KWQATLDADV NRAWFYHSEI SHELDISEGL KSVVDHIKAN   100
GPYDGIVGFS QGAALSSIIT NKISELVPDH PQFKVSVVIS GYSFTEPDPE   150
HPGELRITEK FRDSFAVKPD MKTKMIFIYG ASDQAVPSVR SKYLYDIYLK   200
AQNGNKEKVL AYEHPGGHMV PNKKDIIRPI VEQITSSLQE ASE          243