SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameFET3
Systematic nameYMR058W
DescriptionIron transport multicopper oxidase FET3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP38993
SGD YMR058W
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0004322ferroxidase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006827high affinity iron ion transportIDA:SGD.
Biological processGO:0046688response to copper ionIGI:SGD.
EC number 1.-.-.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
622Phosphoserine.

Sequence Information
Sequence length636
Molecular weight72360

	---------+----------+----------+----------+----------+
MTNALLSIAV LLFSMLSLAQ AETHTFNWTT GWDYRNVDGL KSRPVITCNG   50
QFPWPDITVN KGDRVQIYLT NGMNNTNTSM HFHGLFQNGT ASMDGVPFLT   100
QCPIAPGSTM LYNFTVDYNV GTYWYHSHTD GQYEDGMKGL FIIKDDSFPY   150
DYDEELSLSL SEWYHDLVTD LTKSFMSVYN PTGAEPIPQN LIVNNTMNLT   200
WEVQPDTTYL LRIVNVGGFV SQYFWIEDHE MTVVEIDGIT TEKNVTDMLY   250
ITVAQRYTVL VHTKNDTDKN FAIMQKFDDT MLDVIPSDLQ LNATSYMVYN   300
KTAALPTQNY VDSIDNFLDD FYLQPYEKEA IYGEPDHVIT VDVVMDNLKN   350
GVNYAFFNNI TYTAPKVPTL MTVLSSGDQA NNSEIYGSNT HTFILEKDEI   400
VEIVLNNQDT GTHPFHLHGH AFQTIQRDRT YDDALGEVPH SFDPDNHPAF   450
PEYPMRRDTL YVRPQSNFVI RFKADNPGVW FFHCHIEWHL LQGLGLVLVE   500
DPFGIQDAHS QQLSENHLEV CQSCSVATEG NAAANTLDLT DLTGENVQHA   550
FIPTGFTKKG IIAMTFSCFA GILGIITIAI YGMMDMEDAT EKVIRDLHVD   600
PEVLLNEVDE NEERQVNEDR HSTEKHQFLT KAKRFF                  636