SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameFBA1
Systematic nameYKL060C
DescriptionFructose-bisphosphate aldolase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP14540
SGD YKL060C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004332fructose-bisphosphate aldolase activityIDA:SGD.
Biological processGO:0006094gluconeogenesisIMP:SGD.
Biological processGO:0006096glycolysisIMP:SGD.
EC number 4.1.2.13

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
11Phosphothreonine.
24Phosphothreonine.
56Phosphoserine.
147Phosphoserine.
150Phosphothreonine.
179Phosphothreonine.
268Phosphoserine.
270Phosphoserine.
271Phosphothreonine.
277Phosphothreonine.
310Phosphotyrosine.
313Phosphoserine.

Sequence Information
Sequence length359
Molecular weight39621

	---------+----------+----------+----------+----------+
MGVEQILKRK TGVIVGEDVH NLFTYAKEHK FAIPAINVTS SSTAVAALEA   50
ARDSKSPIIL QTSNGGAAYF AGKGISNEGQ NASIKGAIAA AHYIRSIAPA   100
YGIPVVLHSD HCAKKLLPWF DGMLEADEAY FKEHGEPLFS SHMLDLSEET   150
DEENISTCVK YFKRMAAMDQ WLEMEIGITG GEEDGVNNEN ADKEDLYTKP   200
EQVYNVYKAL HPISPNFSIA AAFGNCHGLY AGDIALRPEI LAEHQKYTRE   250
QVGCKEEKPL FLVFHGGSGS TVQEFHTGID NGVVKVNLDT DCQYAYLTGI   300
RDYVLNKKDY IMSPVGNPEG PEKPNKKFFD PRVWVREGEK TMGAKITKSL   350
ETFRTTNTL                                                359