SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameFAR1
Systematic nameYJL157C
DescriptionCyclin-dependent kinase inhibitor FAR1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1  CDC34RINGSCFCDC4   
2  CDC34RINGSCFCDC4   
3 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
Methodautoradiography (in vitro)
Reference"Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast."
Henchoz S, Chi Y, Catarin B, Herskowitz I, Deshaies RJ, Peter M.
Genes Dev. 11(22):3046-60 (1997) [9367986]
[2]EvidenceHigh MW Product Confirmation
Methodautoradiography (in vitro)
Reference"Nuclear-specific degradation of Far1 is controlled by the localization of the F-box protein Cdc4."
Blondel M, Galan JM, Chi Y, Lafourcade C, Longaretti C, Deshaies RJ, Peter M.
EMBO J. 19(22):6085-97 (2000) [11080155]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP21268
SGD YJL157C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0043332mating projection tipIDA:SGD.
Cellular componentGO:0016020membraneIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0004861cyclin-dependent protein kinase inhibitor a...IDA:SGD.
Biological processGO:0007050cell cycle arrestIMP:SGD.
Biological processGO:0000750pheromone-dependent signal transduction dur...IGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
87Phosphoserine, by CDC28 (Probable).
98Phosphoserine.
110Phosphoserine.
114Phosphoserine.
306Phosphothreonine.

Sequence Information
Sequence length830
Molecular weight94572

	---------+----------+----------+----------+----------+
MKTPTRVSFE KKIHTPPSGD RDAERSPPKK FLRGLSGKVF RKTPEFKKQQ   50
MPTFGYIEES QFTPNLGLMM SKRGNIPKPL NLSKPISPPP SLKKTAGSVA   100
SGFSKTGQLS ALQSPVNITS SNKYNIKATN LTTSLLRESI SDSTTMCDTL   150
SDINLTVMDE DYRIDGDSYY EEDSPTFMIS LERNIKKCNS QFSPKRYIGE   200
KCLICEESIS STFTGEKVVE STCSHTSHYN CYLMLFETLY FQGKFPECKI   250
CGEVSKPKDK DIVPEMVSKL LTGAGAHDDG PSSNMQQQWI DLKTARSFTG   300
EFPQFTPQEQ LIRTADISCD GFRTPRLSNS NQFEAVSYLD SPFLNSPFVN   350
KMATTDPFDL SDDEKLDCDD EIDESAAEVW FSKTGGEHVM VSVKFQEMRT   400
SDDLGVLQDV NHVDHEELEE REKEWKKKID QYIETNVDKD SEFGSLILFD   450
KLMYSDDGEQ WVDNNLVILF SKFLVLFDFE EMKILGKIPR DQFYQVIKFN   500
EDVLLCSLKS TNIPEIYLRF NENCEKWLLP KWKYCLENSS LETLPLSEIV   550
STVKELSHVN IIGALGAPPD VISAQSHDSR LPWKRLHSDT PLKLIVCLNL   600
SHADGELYRK RVLKSVHQIL DGLNTDDLLG IVVVGRDGSG VVGPFGTFIG   650
MINKNWDGWT TFLDNLEVVN PNVFRDEKQQ YKVTLQTCER LASTSAYVDT   700
DDHIATGYAK QILVLNGSDV VDIEHDQKLK KAFDQLSYHW RYEISQRRMT   750
PLNASIKQFL EELHTKRYLD VTLRLPQATF EQVYLGDMAA GEQKTRLIMD   800
EHPHSSLIEI EYFDLVKQQR IHQTLEVPNL                          830