SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameFAA1
Systematic nameYOR317W
DescriptionLong-chain-fatty-acid--CoA ligase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP30624
SGD YOR317W
Gene Ontology (GO)
Cellular componentGO:0005811lipid particleIDA:SGD.
Cellular componentGO:0005741mitochondrial outer membraneIDA:SGD.
Molecular functionGO:0004467long-chain-fatty-acid-CoA ligase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006629lipid metabolic processIGI:SGD.
Biological processGO:0006869lipid transportIGI:SGD.
Biological processGO:0006499N-terminal protein myristoylationIGI:SGD.
EC number 6.2.1.3

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length700
Molecular weight77866

	---------+----------+----------+----------+----------+
MVAQYTVPVG KAANEHETAP RRNYQCREKP LVRPPNTKCS TVYEFVLECF   50
QKNKNSNAMG WRDVKEIHEE SKSVMKKVDG KETSVEKKWM YYELSHYHYN   100
SFDQLTDIMH EIGRGLVKIG LKPNDDDKLH LYAATSHKWM KMFLGAQSQG   150
IPVVTAYDTL GEKGLIHSLV QTGSKAIFTD NSLLPSLIKP VQAAQDVKYI   200
IHFDSISSED RRQSGKIYQS AHDAINRIKE VRPDIKTFSF DDILKLGKES   250
CNEIDVHPPG KDDLCCIMYT SGSTGEPKGV VLKHSNVVAG VGGASLNVLK   300
FVGNTDRVIC FLPLAHIFEL VFELLSFYWG ACIGYATVKT LTSSSVRNCQ   350
GDLQEFKPTI MVGVAAVWET VRKGILNQID NLPFLTKKIF WTAYNTKLNM   400
QRLHIPGGGA LGNLVFKKIR TATGGQLRYL LNGGSPISRD AQEFITNLIC   450
PMLIGYGLTE TCASTTILDP ANFELGVAGD LTGCVTVKLV DVEELGYFAK   500
NNQGEVWITG ANVTPEYYKN EEETSQALTS DGWFKTGDIG EWEANGHLKI   550
IDRKKNLVKT MNGEYIALEK LESVYRSNEY VANICVYADQ SKTKPVGIIV   600
PNHAPLTKLA KKLGIMEQKD SSINIENYLE DAKLIKAVYS DLLKTGKDQG   650
LVGIELLAGI VFFDGEWTPQ NGFVTSAQKL KRKDILNAVK DKVDAVYSSS   700