SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameERG6
Systematic nameYML008C
AliasISE1, LIS1, SED6
DescriptionSterol 24-C-methyltransferase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP25087
SGD YML008C
Gene Ontology (GO)
Cellular componentGO:0005811lipid particleIDA:SGD.
Cellular componentGO:0005741mitochondrial outer membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number 2.1.1.41

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.

Sequence Information
Sequence length383
Molecular weight43431

	---------+----------+----------+----------+----------+
MSETELRKRQ AQFTRELHGD DIGKKTGLSA LMSKNNSAQK EAVQKYLRNW   50
DGRTDKDAEE RRLEDYNEAT HSYYNVVTDF YEYGWGSSFH FSRFYKGESF   100
AASIARHEHY LAYKAGIQRG DLVLDVGCGV GGPAREIARF TGCNVIGLNN   150
NDYQIAKAKY YAKKYNLSDQ MDFVKGDFMK MDFEENTFDK VYAIEATCHA   200
PKLEGVYSEI YKVLKPGGTF AVYEWVMTDK YDENNPEHRK IAYEIELGDG   250
IPKMFHVDVA RKALKNCGFE VLVSEDLADN DDEIPWYYPL TGEWKYVQNL   300
ANLATFFRTS YLGRQFTTAM VTVMEKLGLA PEGSKEVTAA LENAAVGLVA   350
GGKSKLFTPM MLFVARKPEN AETPSQTSQE ATQ                     383