SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameERG5
Systematic nameYMR015C
AliasCYP61
DescriptionCytochrome P450 61

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K164;K198        
2         
3 MULTI       
4        Proteasomal Degradation
5K198        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[5]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP54781
SGD YMR015C
Gene Ontology (GO)
EC number 1.14.14.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length538
Molecular weight61334

	---------+----------+----------+----------+----------+
MSSVAENIIQ HATHNSTLHQ LAKDQPSVGV TTAFSILDTL KSMSYLKIFA   50
TLICILLVWD QVAYQIKKGS IAGPKFKFWP IIGPFLESLD PKFEEYKAKW   100
ASGPLSCVSI FHKFVVIAST RDLARKILQS SKFVKPCVVD VAVKILRPCN   150
WVFLDGKAHT DYRKSLNGLF TKQALAQYLP SLEQIMDKYM DKFVRLSKEN   200
NYEPQVFFHE MREILCALSL NSFCGNYITE DQVRKIADDY YLVTAALELV   250
NFPIIIPYTK TWYGKKTADM AMKIFENCAQ MAKDHIAAGG KPVCVMDAWC   300
KLMHDAKNSN DDDSRIYHRE FTNKEISEAV FTFLFASQDA SSSLACWLFQ   350
IVADRPDVLA KIREEQLAVR NNDMSTELNL DLIEKMKYTN MVIKETLRYR   400
PPVLMVPYVV KKNFPVSPNY TAPKGAMLIP TLYPALHDPE VYENPDEFIP   450
ERWVEGSKAS EAKKNWLVFG CGPHVCLGQT YVMITFAALL GKFALYTDFH   500
HTVTPLSEKI KVFATIFPKD DLLLTFKKRD PITGEVFE                538

	* Ubiquitinated lysine is denoted as red color.