SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameERG3
Systematic nameYLR056W
AliasPSO6, SYR1
DescriptionC-5 sterol desaturase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K324;K344        
2 MULTI    RPN10 Proteasomal Degradation
3        Proteasomal Degradation
4K324        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[4]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32353
SGD YLR056W
Gene Ontology (GO)
Biological processGO:0006897endocytosisIGI:SGD.
EC number 1.3.3.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length365
Molecular weight42730

	---------+----------+----------+----------+----------+
MDLVLEVADH YVLDDLYAKV LPASLAANIP VKWQKLLGLN SGFSNSTILQ   50
ETLNSKNAVK ECRRFYGQVP FLFDMSTTSF ASLLPRSSIL REFLSLWVIV   100
TIFGLLLYLF TASLSYVFVF DKSIFNHPRY LKNQMAMEIK LAVSAIPWMS   150
MLTVPWFVME LNGHSKLYMK IDYENHGVRK LIIEYFTFIF FTDCGVYLAH   200
RWLHWPRVYR ALHKPHHKWL VCTPFASHSF HPVDGFLQSI SYHIYPLILP   250
LHKVSYLILF TFVNFWTVMI HDGQYLSNNP AVNGTACHTV HHLYFNYNYG   300
QFTTLWDRLG GSYRRPDDSL FDPKLRDAKE TWDAQVKEVE HFIKEVEGDD   350
NDRIYENDPN TKKNN                                         365

	* Ubiquitinated lysine is denoted as red color.