SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameERG13
Systematic nameYML126C
AliasHMGS
DescriptionHydroxymethylglutaryl-CoA synthase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP54839
SGD YML126C
Gene Ontology (GO)
Molecular functionGO:0004421hydroxymethylglutaryl-CoA synthase activityIMP:SGD.
Biological processGO:0006696ergosterol biosynthetic processIMP:SGD.
EC number 2.3.3.10

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length491
Molecular weight55014

	---------+----------+----------+----------+----------+
MKLSTKLCWC GIKGRLRPQK QQQLHNTNLQ MTELKKQKTA EQKTRPQNVG   50
IKGIQIYIPT QCVNQSELEK FDGVSQGKYT IGLGQTNMSF VNDREDIYSM   100
SLTVLSKLIK SYNIDTNKIG RLEVGTETLI DKSKSVKSVL MQLFGENTDV   150
EGIDTLNACY GGTNALFNSL NWIESNAWDG RDAIVVCGDI AIYDKGAARP   200
TGGAGTVAMW IGPDAPIVFD SVRASYMEHA YDFYKPDFTS EYPYVDGHFS   250
LTCYVKALDQ VYKSYSKKAI SKGLVSDPAG SDALNVLKYF DYNVFHVPTC   300
KLVTKSYGRL LYNDFRANPQ LFPEVDAELA TRDYDESLTD KNIEKTFVNV   350
AKPFHKERVA QSLIVPTNTG NMYTASVYAA FASLLNYVGS DDLQGKRVGL   400
FSYGSGLAAS LYSCKIVGDV QHIIKELDIT NKLAKRITET PKDYEAAIEL   450
RENAHLKKNF KPQGSIEHLQ SGVYYLTNID DKFRRSYDVK K            491