SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameENO2
Systematic nameYHR174W
AliasENOB
DescriptionEnolase 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP00925
SGD YHR174W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0000015phosphopyruvate hydratase complexIDA:SGD.
Cellular componentGO:0005625soluble fractionIDA:SGD.
Cellular componentGO:0000324vacuole, cell cycle-correlated morphologyIDA:SGD.
Molecular functionGO:0004634phosphopyruvate hydratase activityIMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006094gluconeogenesisIEP:SGD.
Biological processGO:0006096glycolysisIMP:SGD.
Biological processGO:0032889regulation of vacuole fusion, non-autophagicIDA:SGD.
EC number 4.2.1.11

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
10Phosphoserine.
40Phosphoserine.
188Phosphoserine.
205Phosphoserine.
221Phosphothreonine.
373Phosphoserine.

Sequence Information
Sequence length437
Molecular weight46914

	---------+----------+----------+----------+----------+
MAVSKVYARS VYDSRGNPTV EVELTTEKGV FRSIVPSGAS TGVHEALEMR   50
DEDKSKWMGK GVMNAVNNVN NVIAAAFVKA NLDVKDQKAV DDFLLSLDGT   100
ANKSKLGANA ILGVSMAAAR AAAAEKNVPL YQHLADLSKS KTSPYVLPVP   150
FLNVLNGGSH AGGALALQEF MIAPTGAKTF AEAMRIGSEV YHNLKSLTKK   200
RYGASAGNVG DEGGVAPNIQ TAEEALDLIV DAIKAAGHDG KVKIGLDCAS   250
SEFFKDGKYD LDFKNPESDK SKWLTGVELA DMYHSLMKRY PIVSIEDPFA   300
EDDWEAWSHF FKTAGIQIVA DDLTVTNPAR IATAIEKKAA DALLLKVNQI   350
GTLSESIKAA QDSFAANWGV MVSHRSGETE DTFIADLVVG LRTGQIKTGA   400
PARSERLAKL NQLLRIEEEL GDKAVYAGEN FHHGDKL                 437