SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameELA1
Systematic nameYNL230C
DescriptionElongin-A

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53861
SGD YNL230C
Gene Ontology (GO)
Cellular componentGO:0031463Cul3-RING ubiquitin ligase complexIMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0042787protein ubiquitination during ubiquitin-dep...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
278Phosphoserine.

Sequence Information
Sequence length379
Molecular weight43981

	---------+----------+----------+----------+----------+
MKSLQTLCEI SLMRNHSNIQ SVSNVPYHLL KRILQKVKIP QLLKLEKSNV   50
LLIFDDDELW LEFLRQDFPT NVHEQFVSKR DIICKYYFDF VKENDIELYH   100
SNQDLLKSCV RQSVVKDIRN NKYRIPYRML YSKYQQEVEK KQEESAERLR   150
LEMQKLQQER EKKQTIVVDH TVYFKKRNTK KTTRLHNEPH SQLYMKSLKD   200
HESRLKHFKD GGFNIAKRHA QRVAFGGQAG GQSSSPKKGP LSIKPEPVKV   250
NRQMDNVTAE KKDVTQPITP VKKRRSESPS IFLNRKKPAL FRPTPKTNAA   300
GSRPHTTAIT NDHRTTSHPY PHKDVVTSIS SVTANPVTKG HKKKKSGIFV   350
RNAGSDGDSF PHVTATGPTT RPYIYEPRK                          379