SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameEEB1
Systematic nameYPL095C
DescriptionMedium-chain fatty acid ethyl ester synthase/esterase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ02891
SGD YPL095C
Gene Ontology (GO)
Molecular functionGO:0004026alcohol O-acetyltransferase activityIDA:SGD.
Molecular functionGO:0016788hydrolase activity, acting on ester bondsIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0051791medium-chain fatty acid metabolic processIMP:SGD.
EC number 2.3.1.84,3.1.1.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length456
Molecular weight51724

	---------+----------+----------+----------+----------+
MFRSGYYPTV TPSHWGYNGT VKHVLGEKGT KSLAFRDSKR QIPLHEFVTK   50
HVPTLKDGAN FRLNSLLFTG YLQTLYLSAG DFSKKFQVFY GREIIKFSDG   100
GVCTADWVMP EWEQTYSLNA EKASFNEKQF SNDEKATHPK GWPRLHPRTR   150
YLSSEELEKC HSKGYSYPLV VVLHGLAGGS HEPLIRALSE DLSKVGDGKF   200
QVVVLNARGC SRSKVTTRRI FTALHTGDVR EFLNHQKALF PQRKIYAVGT   250
SFGAAMLTNY LGEEGDNCPL NAAVALSNPW DFVHTWDKLA HDWWSNHIFS   300
RTLTQFLTRT VKVNMNELQV PENFEVSHKP TVEKPVFYTY TRENLEKAEK   350
FTDILEFDNL FTAPSMGLPD GLTYYRKASS INRLPNIKIP TLIINATDDP   400
VTGENVIPYK QARENPCVLL CETDLGGHLA YLDNESNSWL TKQAAEFLGS   450
FDELVL                                                   456