SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameECM3
Systematic nameYOR092W
DescriptionProtein ECM3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ99252
SGD YOR092W
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumIDA:SGD.
Biological processGO:0007047cell wall organization and biogenesisIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length613
Molecular weight68015

	---------+----------+----------+----------+----------+
MTHITLGQAI WASVRPIIKI YLIIGVGFGL CKMNILTVQA TRSISDIVLT   50
ILLPCLSFNK IVANIEDNDI KDVGIICLTS VILFATGLGF AFIVRSVLPV   100
PKRWRGGILA GGMFPNISDL PIAYLQSMDQ GFIFTEAEGE KGVANVIIFL   150
AMFLICVFNL GGFRLIENDF HYKGDDDEEN TLTNDDSAQQ PTQPIEGNSS   200
SSSNQDILKE PNESTVPNSS QASYISEKNK KEKTELSVPK PTHTAPPAID   250
DRSSNSSAVV SIDSITHSLR TNHVDAQSVS ELNDPTYRTR SQPIAYTTES   300
RTSHVHNNRR NSITGSLRSI DMRELPAEGM SDLIREYSNV DQYGRRRKSS   350
ISSQGAPSVL QADGTISPNL TRTSTLQRVK TSNLTRIITS DATVSKKDIE   400
TSGSSLPKWL QKFPLTKFFV FFLKNCLRPC SMAVILALII AFIPWVKALF   450
VTTSNTPKIK QAPDNAPALT FIMDFTSYVG AASVPFGLIL LGATLGRLKI   500
GKLYPGFWKS AVVLVFLRQC IMPIFGVLWC DRLVKAGWLN WENDKMLLFV   550
TAITWNLPTM TTLIYFTASY TPEDETEPVQ MECTSFFLML QYPLMVVSLP   600
FLVSYFIKVQ MKL                                           613