SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameECM29
Systematic nameYHL030W
DescriptionProteasome component ECM29

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38737
SGD YHL030W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0000502proteasome complex (sensu Eukaryota)IDA:SGD.
Molecular functionGO:0005515protein bindingIDA:SGD.
Biological processGO:0030163protein catabolic processIC:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1868
Molecular weight210431

	---------+----------+----------+----------+----------+
MSISSDEAKE KQLVEKAELR LAIADSPQKF ETNLQTFLPP LLLKLASPHA   50
SVRTAVFSAL KNLISRINTL PQVQLPVRAL IVQAKEPNLA AQQDSTNVRL   100
YSLLLASKGI DRLSLQDRQQ LLPLVVSNIS CLTGTVAARM FHILLKLILE   150
WVAPQESSHE QEEFVQFLQL DNDGFSFLMR QFTRFFLLVP SKQVQVSQQP   200
LSRGYTCPGL SLTDVAFFTY DAGVTFNKEQ LNKFKKAIFQ FVCRGMAATQ   250
TIEQSPRMIE LMEFLCVVST DSTNLSDDAA QFMKRFPMPY ENEEFITFLQ   300
TLYIGNTANG RPPVKAILQE KILSILNRSH FATTKAECIS LICSIGLHSS   350
EYKLRSLTLS FIRHVAKLNY KNLNPASSSP SSTDFSTCIV SLIRNNLHAE   400
GWPKLQLGPQ TPAFNTAILQ RQLQYETLGD ILKRDFELVS DLSYIEFLFE   450
SLKNDLPQFR SSIQESLLSL VGHLSILPQQ SKLKLKNLLR KNLSIDEQQR   500
EDNNDAVNSI MALKFVSIKF TNAAFPFHDP EARLFNIWGT VRTNRFDIIE   550
ESFKGLQPFW FRVNNASINT SATVKTSDLL GSHLSETEFP PFREFLQVLI   600
DQLDSEAASI TRKSLNNAVR FSKQCLISNA IYGKKTMVIQ DEDWSVRIDK   650
ALELDDTVVS RVNEMVQGMN DDIFIRYLTL LSNEFTATNS KGEQIAIFPY   700
QDPIFGSVLL TLLNFVSNNV LRRLEILVPD LYHLVIMKFQ SLSDNDLAVC   750
ATIIGIISTA IADSTHVKRI TKIAQSQTMA ETYVASYVVP RLYLKDQTNH   800
IESDSILNLL NILTTHLSHP GTNKDMILKL VCQVTKFGLL LQVSAQERKD   850
FLKKVMDTIQ DKLINDVTAI QTWSYLSLYS TDLENSSLFQ EKLLETNVSK   900
QNDFLFSVGE SLSVVAGKWS SKYLIKQIDI PNFNVEIMQQ KFPATNVTTI   950
LDEIFSGCDS TKPSLRKASC IWLLSYIQYL GHLPEVSSKC NDIHLRFMRF   1000
LADRDEFIQD SAARGLSLVY EIGGSDLKES MVKGLLKSFT ESTAGSASTS   1050
ATGISGSVSE ETELFEPGVL NTGDGSISTY KDILNLASEV GDPALVYKFM   1100
SLAKSSALWS SRKGIAFGLG AIMSKSSLEE LLLKDQQTAK KLIPKLYRYR   1150
FDPFQAVSRS MTDIWNTLIP ESSLTISLYF NDILDELLCG MANKEWRVRE   1200
ASTSALLQLI QSQPQEKFSE KMLKIWTMAF RTMDDIKDSV REVGTKFTTV   1250
LAKILARSID VEKGVNPTKS KEILDNILPF LWGPHGLNSD AEEVRNFALT   1300
TLIDLVKHSP GAIKPFTPKL IYDFITLFSS IEPQVINYLA LNAANYNIDA   1350
NVIDTQRKNG VTNSPLFQTI EKLINNSDDC MMEEIINVVI KASRKSVGLP   1400
SKVASSLVII ILVKRYSIEM KPYSGKLLKV CLTMFEDRNE SVNIAFAISM   1450
GYLFKVSALD KCIKYSEKLI TKYFEPTSTE NNKKVVGTAI DSILNYAKSE   1500
FDNVASVFMP LIFIACNDED KDLETLYNKI WTEASSSGAG TVKLYLPEIL   1550
NVLCVNIKSN DFSIRKTCAK SVIQLCGGIN DSIPYPQIVK LFDISREALS   1600
GRSWDGKEHI VAALVSLTEK FSQTVADNND LQESINHVMY TEVSRKSMKY   1650
VKKILPLYAR YINVNPQEET ITFLIEKAKE MIRLLGSESD DSEGSIKQTS   1700
DESTIKRIKP NTEITQKSSK ENIENEEYVI NLLKVSVDIC NNSKSRYPMN   1750
LLEFIIDEIA YLFHNDRIIH TWRTQLAASE IGISIVGRFS TISSADFIQN   1800
VGRLWDQTFP INCNKETIEN VKLQMIKFGG LIIQKIPSLQ NNIEENLRLL   1850
NSIDSTSRIE LELKNIGL                                      1868