SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameECM21
Systematic nameYBL101C
DescriptionProtein ECM21

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K577;K651;K712;K794;K807;K1024        
2K577;K651;K712;K794;K807;K1024        
3         
4 MULTI    RPN10 Proteasomal Degradation
5 MULTI(K63-linked) HECTNARSP5 UBP2 
6        Proteasomal Degradation
7K191;K577;K651;K712;K807        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS
Reference"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]
[3]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[5]EvidenceUb-conjugate Confirmation
Methodautoradiography (in vitro)
Reference"The deubiquitinating enzyme Ubp2 modulates Rsp5-dependent Lys63-linked polyubiquitin conjugates in Saccharomyces cerevisiae."
Kee Y, Munoz W, Lyon N, Huibregtse JM.
J Biol Chem. 281(48):36724-31 (2006) [17028178]
[6]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[7]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38167
SGD YBL101C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Biological processGO:0007047cell wall organization and biogenesisIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
13Phosphoserine.
18Phosphoserine.
56Phosphoserine.
87Phosphothreonine.
123Phosphothreonine.
126Phosphoserine.
127Phosphoserine.
140Phosphoserine.
300Phosphoserine.
306Phosphoserine.
308Phosphoserine.
527Phosphoserine.
550Phosphoserine.
775Phosphoserine.
962Phosphoserine.
1030Phosphoserine.
1035Phosphoserine.
1038Phosphoserine.
1039Phosphoserine.
1041Phosphothreonine.

Sequence Information
Sequence length1117
Molecular weight123610

	---------+----------+----------+----------+----------+
MPFITSRPVA KNSSHSLSET DLNQSKGQPF QPSPTKKLGS MQQRRRSSTI   50
RHALSSLLGG ANVHSPAVLN NTTKGGNNNG NIRSSNTDAQ LLGKKQNKQP   100
PPNARRHSTT AIQGSISDSA TTTPRSSTSD TNRRTSGRLS VDQEPRISGG   150
RYSQIEEDST VLDFDDDHNS SAVVSSDLSS TSLTRLANSK KFNEQFLIEY   200
LTARGLLGPK TVLSNEYLKI SISTSGESVF LPTISSNDDE YLSRLNGLND   250
GTDDAEADFF MDGIDQQEGN TPSLATTAAA TESGGSINEN RDTLLRENNS   300
GDHPGSGSEL NTRSVEIDSS MVSYSIAVIV SVKKPTRFTD MQLELCSRVK   350
VFWNTGVPPT KTFNEEFYNA ASMKWNLNDE NFDLFVPLSI SPDDQMIENN   400
SNDRQMRLFK NIPTEERLYL DKTKTKASLL NAIDVNKTHL YQPGDYVFLV   450
PVVFSNHIPE TIYLPSARVS YRLRLATKAI NRKGFYRQDS NSPQPIVSPD   500
SSSSLSSTTS SLKLTETESA QAHRRISNTL FSKVKNHLHM SSHQLKNEES   550
GEEDIFAEYP IKVIRTPPPV AVSTANKPIY INRVWTDSLS YEISFAQKYV   600
SLNSEVPIKI KLAPICKNVC VKRIHVSITE RVTFVSKGYE YEYDQTDPVA   650
KDPYNPYYLD FASKRRKERS VSLFEIRTKE KGTRALREEI VENSFNDNLL   700
SYSPFDDDSD SKGNPKERLG ITEPIIIETK LKFPKYEDLD KRTAKIIPPY   750
GIDAYTSIPN PEHAVANGPS HRRPSVIGFL SGHKGSKSHE ENEKPVYDPK   800
FHQTIIKSNS GLPVKTHTRL NTPKRGLYLD SLHFSNVYCR HKLEIMLRIS   850
KPDPECPSKL RHYEVLIDTP IFLVSEQCNS GNMELPTYDM ATMEGKGNQV   900
PLSMNSDFFG NTCPPPPTFE EAISVPASPI VSPMGSPNIM ASYDPDLLSI   950
QQLNLSRTTS VSGPSGYSDD AGVPNVNRNS ISNANAMNGS ISNSAFVSGN   1000
SGQGVARARA TSVNDRSRFN NLDKLLSTPS PVNRSHNSSP TNGLSQANGT   1050
VRIPNATTEN SKDKQNEFFK KGYTLANVKD DEEQEGIVSS SSADSLLSHG   1100
NEPPRYDEIV PLMSDEE                                       1117

	* Ubiquitinated lysine is denoted as red color.