SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameECM14
Systematic nameYHR132C
DescriptionProtein ECM14

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP38836
SGD YHR132C
Gene Ontology (GO)
Cellular componentGO:0000324vacuole, cell cycle-correlated morphologyIDA:SGD.
Biological processGO:0007047cell wall organization and biogenesisIMP:SGD.
EC number 3.4.17.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length430
Molecular weight49829

	---------+----------+----------+----------+----------+
MLHMNSLWGC FLFVLLAVTG AVQGLQEDYS EYAVYRFTSD NYSTLVRDVI   50
APLTDDYDVW TRSNNFIDIK LPKEIGEQIN DGQVIIDNMN ELIQNTLPTS   100
QMMAREQAVF ENDYDFFFNE YRDLDTIYMW LDLLERSFPS LVAVEHLGRT   150
FEGRELKALH ISGNKPESNP EKKTIVITGG IHAREWISVS TVCWALYQLL   200
NRYGSSKKET KYLDDLDFLV IPVFNPDGYA YTWSHDRLWR KNRQRTHVPQ   250
CLGIDIDHSF GFQWEKAHTH ACSEEYSGET PFEAWEASAW YKYINETKGD   300
YKIYGYIDMH SYSQEILYPY AYSCDALPRD LENLLELSYG LSKAIRSKSG   350
RNYDVISACK DRGSDIFPGL GAGSALDFMY HHRAHWAFQL KLRDTGNHGF   400
LLPPENIKPV GKETYAALKY FCDFLLDPEI                          430