SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameEAR1
Systematic nameYMR171C
DescriptionProtein EAR1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2   HECTNARSP5   
3K384        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ03212
SGD YMR171C
Gene Ontology (GO)
Cellular componentGO:0005768endosomeIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
516Phosphoserine.
519Phosphoserine.

Sequence Information
Sequence length550
Molecular weight62532

	---------+----------+----------+----------+----------+
MSFKFLIESL LLGSISGQIR CGRSSVIPRG DVSYGGDDTD ELNMDIMLFA   50
FGTLIVVYIV ICIVYFFTKQ IATRLITAYY NEHGPGQRIS LFSDYDENNA   100
HVHSRRLMEN MSLRWPNNLD DADEVRDKLA QLSPEEQFYY KQGEEYIKQN   150
PPFLLNQGLL QQSEDSNPDT TREDPIMNEQ TRQYIQEEGA YAWEFSPNPD   200
MPNHTVIVEN KTEVSFLNYN YDASISTNLP IPCINKVYYC EFKIFETDGP   250
LNSDENVSKG VISFGLSTQP YPYFRLPGRH HHSIAYDSNG ARRFNDSFKL   300
NEQLRTLFPQ CEKGDIVGIG YRSRSGTVFF TRNGKKLNEK SVGGHIRGWK   350
FQYLYPIIGS NVPCQIHVNF GTYGFVYIEA NVKKWGYAKS NGIKLPPPSY   400
EDYGKDTLLE SGGEDNDFDE DFSDGDSDNI AAGSTTNLND DIIIRNGEIL   450
PPPPGFEFTM SPPTGKKIIN EEINLDSLPM LPPSYSDDEH HSKNDKSAIS   500
GRIIGTSRNL ITDEASFDSV DNDNEDENDH ERDPEQFSEF DDYESRMHGI   550

	* Ubiquitinated lysine is denoted as red color.