SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameEAF7
Systematic nameYNL136W
DescriptionChromatin modification-related protein EAF7

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI HECTNARSP5   
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
Methodluminescent assay (biotin-ubiquitin, GST-substrate)
Reference"A high throughput screen to identify substrates for the ubiquitin ligase Rsp5."
Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, Lee T, Chan D, Wolting C, Edwards A, Bosse R, Rotin D.
J Biol Chem. 280(33):29470-8 (2005) [15955809]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53911
SGD YNL136W
Gene Ontology (GO)
Cellular componentGO:0035267NuA4 histone acetyltransferase complexIPI:SGD.
Biological processGO:0006281DNA repairIDA:SGD.
Biological processGO:0016573histone acetylationIPI:SGD.
Biological processGO:0006357regulation of transcription from RNA polyme...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
225Phosphoserine.
393Phosphoserine.
397Phosphoserine.
411Phosphoserine.

Sequence Information
Sequence length425
Molecular weight49391

	---------+----------+----------+----------+----------+
MVVHWTIVDE IRLLRWASEF KPAGIHKHFH MFCIVERMNS PDKYPVTLLQ   50
KETMKLGKVF TAKDIWDKLS QSYNLEKIDE MENTYSLEAT TESSRNGNGN   100
GDDAEIHEET LLELNNRIRV RKQDFTLPWE EYGELILENA RKSPNSNEEY   150
PRVEDMNEKD STIPKESPST DLKNDNNKQE KNATIKVKEL PEYHTEENDS   200
PIDVQKEPIK EVQSDEKELQ REHMSEEEQK MKSTNKTAAP VRKSQRLKRS   250
KEVKFEDEEK EEIEEDNTKD EEQKEKKEEI QEPKITHNEE VDKEKNENEE   300
GDDEREKSTS YENTNGSESE GVDEGVDEEL GYESEREAEG KGKQIESEGG   350
NLKKKTENKK GDDQQDDTKK DSKDKNEPLA KRTRHSSSTG NTSNETSPKR   400
KRRKAGSRKN SPPATRVSSR LRNKK                              425