SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDSE4
Systematic nameYNR067C
AliasENG1
DescriptionEndo-1,3(4)-beta-glucanase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53753
SGD YNR067C
Gene Ontology (GO)
Cellular componentGO:0030428cell septumIDA:SGD.
Cellular componentGO:0009277chitin- and beta-glucan-containing cell wallIDA:SGD.
Biological processGO:0007109cytokinesis, completion of separationIMP:SGD.
EC number 3.2.1.6

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1117
Molecular weight121064

	---------+----------+----------+----------+----------+
MQLYLTLLFL LSFVECSYIS FISNNADEIL ETDLIETLSY ATLTVGEPYV   50
AQSVVVTRVS AASHSPLSVS PKNRVSASPI NSQDSDSNTR TAVQLSLSLS   100
NYASQVSQKI SAQTNNDPVT VSNIYANDNS KSKSSVHNLS SVSGVASVMP   150
SASTMRKVTT LLSQTASTST STLFSSSLSI SGTQLNGTLL TSVSKGTIDP   200
LVTQMPSYSS QETKIIPSSL TSNKTIYTIS VRTNAATATG EDSFIASTPA   250
SSTLFYPSNS TQDLVQTLAS TTASPAYPSN RTQITLSPSV SLYSTTSPIY   300
PSNITENGSS PSPSLSSTVS PVYPSSSTGN ILLSSLFSTV DSSSSPVSST   350
LDTIYVSSSM QATISSSSSS RQTKTSSSSL STSTSSTATT TENSSTTTIV   400
NLFNAVSTDE PPTVFDRSPN PMSLADGVSN DGPIQTNKFY TNLIVGSQES   450
PAFVYPYSLW KYTSSSYGFA VQHTTVDQYS YGGYDSSGNA EYLVNPLGIA   500
HVVFSASNFD SSMTMQVDEM TLSSTRVVLS ESNDSSNYLE IPLVQGMGFA   550
TGIYHGSLNA KIGSSVGFNT IVSESSSNLA QGILKYRITL LNGVTWLCYV   600
IGPDDLTSTD FSLEVSSEYE IKASASVDGL IIQLAVAPSE TDYEVFYDQA   650
AGMYVTNFKL QGVSDGSTAT YEFSYTTQGE SASGSTMIFA LPHHESSFSD   700
IMQDYYTGIQ LASTTKGVMN GYLTTSLQFS TSLNRQISWL PWSSQLGSNL   750
LEYSKEQLQL LAEVANSELQ VSISESISGL NTYYLGKVID KYSYILLTVS   800
EIIQDEASTK STLENIKSAF DILLQNEQTY PLIYDTKFNG LVSSGDWGST   850
STQYDFGNTY YNDHHFHYGY IIHAAAVIGY VDSKLNGTWA ADNKDWVNSL   900
VRDVANPSEK DEYFAQSRMF DWFNGHSWAA GLYENGNGKN EESSSEDYNF   950
AYAMKLWGAT IGDQSMELRG DLMISIMKDA MNDYFYYQND NTVEPEEIIG   1000
NKVSGILFDN IIDYTTYFGT NTEYIHGIHM LPITPVSSNI RSETFVEEEW   1050
QTKIEPIIES IESGWTGILK LNQALFDPVD SYAFFSDSTF DSSTYLDNGM   1100
SRTWALAFSG GLANSIA                                       1117