SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDSE1
Systematic nameYER124C
DescriptionProtein DSE1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K553        
2 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP40077
SGD YER124C
Gene Ontology (GO)
Cellular componentGO:0005935cellular bud neckIDA:SGD.
Biological processGO:0007047cell wall organization and biogenesisIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length573
Molecular weight63999

	---------+----------+----------+----------+----------+
MQDTKYYEPT NIFRQPAINI KKRSDKKRIL QSMMTLSTYK KTWQNNTSKM   50
NSPILRKASD NFNDYYTTKK LKSDYWKLYG VDESELSIPS DMSIVDNILL   100
VSTMNEKDNL KLFEISAEKK LKELQTITVP GKPITCICLL PMVDFPPQIF   150
PSSQINPNHN QLILTGHQDG IVNLIATSTY KGCAKIIKRF NHNKFLKSTV   200
STSIPILEIT PKTAPILKVS PWNKTGFVSL LNDSLFIYDL KSNLDCIKTP   250
IFLQSYPGIN SFAVNEFHDP FLLALVGSKF GPNGISLLDL RTNLYIPDIL   300
DNSISAGCGK DHLQRKNTSL DCVWISNHHV AQSLNDKIQI WDIQSCDGKP   350
VCELYAKKGY IESLKFNENT GALYSSDDQG FVICWDLQNL QNMKYGELVH   400
GFNSISLDSE SELLLTKQVF QCGNIIVSGM SDKNICLKSN DTKANGKGCG   450
FLFLDMANDG SLVTLDNFCE LGLHQICQVQ FNVDTGKIID GNGTGKSGIS   500
DSSMLSLSNE SDHSMTETSD DMFSNSGNWD CSSANTVSEG RLNDDQEDIV   550
FTKRMYSVND VHLSGSTIDT TVV                                573

	* Ubiquitinated lysine is denoted as red color.