SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDRE2
Systematic nameYKR071C
DescriptionProtein DRE2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP36152
SGD YKR071C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
158Phosphoserine.
186Phosphoserine.
206Phosphoserine.

Sequence Information
Sequence length348
Molecular weight38543

	---------+----------+----------+----------+----------+
MSQYKTGLLL IHPAVTTTPE LVENTKAQAA SKKVKFVDQF LINKLNDGSI   50
TLENAKYETV HYLTPEAQTD IKFPKKLISV LADSLKPNGS LIGLSDIYKV   100
DALINGFEII NEPDYCWIKM DSSKLNQTVS IPLKKKKTNN TKLQSGSKLP   150
TFKKASSSTS NLPSFKKADH SRQPIVKETD SFKPPSFKMT TEPKVYRVVD   200
DLIEDSDDDD FSSDSSKAQY FDQVDTSDDS IEEEELIDED GSGKSMITMI   250
TCGKSKTKKK KACKDCTCGM KEQEENEIND IRSQQDKVVK FTEDELTEID   300
FTIDGKKVGG CGSCSLGDAF RCSGCPYLGL PAFKPGQPIN LDSISDDL     348