SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDPS1
Systematic nameYLL018C
AliasAPS, APS1
DescriptionAspartyl-tRNA synthetase, cytoplasmic

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP04802
SGD YLL018C
Gene Ontology (GO)
Molecular functionGO:0004815aspartate-tRNA ligase activityIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Molecular functionGO:0003723RNA bindingIDA:SGD.
EC number 6.1.1.12

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.
14Phosphoserine.
301Phosphoserine.
546Phosphoserine.

Sequence Information
Sequence length557
Molecular weight63516

	---------+----------+----------+----------+----------+
MSQDENIVKA VEESAEPAQV ILGEDGKPLS KKALKKLQKE QEKQRKKEER   50
ALQLEAEREA REKKAAAEDT AKDNYGKLPL IQSRDSDRTG QKRVKFVDLD   100
EAKDSDKEVL FRARVHNTRQ QGATLAFLTL RQQASLIQGL VKANKEGTIS   150
KNMVKWAGSL NLESIVLVRG IVKKVDEPIK SATVQNLEIH ITKIYTISET   200
PEALPILLED ASRSEAEAEA AGLPVVNLDT RLDYRVIDLR TVTNQAIFRI   250
QAGVCELFRE YLATKKFTEV HTPKLLGAPS EGGSSVFEVT YFKGKAYLAQ   300
SPQFNKQQLI VADFERVYEI GPVFRAENSN THRHMTEFTG LDMEMAFEEH   350
YHEVLDTLSE LFVFIFSELP KRFAHEIELV RKQYPVEEFK LPKDGKMVRL   400
TYKEGIEMLR AAGKEIGDFE DLSTENEKFL GKLVRDKYDT DFYILDKFPL   450
EIRPFYTMPD PANPKYSNSY DFFMRGEEIL SGAQRIHDHA LLQERMKAHG   500
LSPEDPGLKD YCDGFSYGCP PHAGGGIGLE RVVMFYLDLK NIRRASLFPR   550
DPKRLRP                                                  557