SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDPL1
Systematic nameYDR294C
AliasBST1
DescriptionSphingosine-1-phosphate lyase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ05567
SGD YDR294C
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumIDA:SGD.
Molecular functionGO:0008117sphinganine-1-phosphate aldolase activityIDA:SGD.
Biological processGO:0019722calcium-mediated signalingIMP:SGD.
Biological processGO:0009267cellular response to starvationIMP:SGD.
Biological processGO:0006665sphingolipid metabolic processIGI:SGD.
EC number 4.1.2.27

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length589
Molecular weight65566

	---------+----------+----------+----------+----------+
MSGVSNKTVS INGWYGMPIH LLREEGDFAQ FMILTINELK IAIHGYLRNT   50
PWYNMLKDYL FVIFCYKLIS NFFYLLKVYG PVRLAVRTYE HSSRRLFRWL   100
LDSPFLRGTV EKEVTKVKQS IEDELIRSDS QLMNFPQLPS NGIPQDDVIE   150
ELNKLNDLIP HTQWKEGKVS GAVYHGGDDL IHLQTIAYEK YCVANQLHPD   200
VFPAVRKMES EVVSMVLRMF NAPSDTGCGT TTSGGTESLL LACLSAKMYA   250
LHHRGITEPE IIAPVTAHAG FDKAAYYFGM KLRHVELDPT TYQVDLGKVK   300
KFINKNTILL VGSAPNFPHG IADDIEGLGK IAQKYKLPLH VDSCLGSFIV   350
SFMEKAGYKN LPLLDFRVPG VTSISCDTHK YGFAPKGSSV IMYRNSDLRM   400
HQYYVNPAWT GGLYGSPTLA GSRPGAIVVG CWATMVNMGE NGYIESCQEI   450
VGAAMKFKKY IQENIPDLNI MGNPRYSVIS FSSKTLNIHE LSDRLSKKGW   500
HFNALQKPVA LHMAFTRLSA HVVDEICDIL RTTVQELKSE SNSKPSPDGT   550
SALYGVAGSV KTAGVADKLI VGFLDALYKL GPGEDTATK               589