SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDNF1
Systematic nameYER166W
DescriptionProbable phospholipid-transporting ATPase DNF1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32660
SGD YER166W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0004012phospholipid-translocating ATPase activityIGI:SGD.
Biological processGO:0006897endocytosisIGI:SGD.
Biological processGO:0030467establishment and/or maintenance of cell po...IGI:SGD.
Biological processGO:0006886intracellular protein transportIGI:SGD.
Biological processGO:0045332phospholipid translocationIGI:SGD.
EC number 3.6.3.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
53Phosphoserine.
81Phosphoserine.
85Phosphothreonine.
92Phosphoserine.
365Phosphoserine.
1506Phosphoserine.

Sequence Information
Sequence length1571
Molecular weight177798

	---------+----------+----------+----------+----------+
MSGTFHGDGH APMSPFEDTF QFEDNSSNED THIAPTHFDD GATSNKYSRP   50
QVSFNDETPK NKREDAEEFT FNDDTEYDNH SFQPTPKLNN GSGTFDDVEL   100
DNDSGEPHTN YDGMKRFRMG TKRNKKGNPI MGRSKTLKWA RKNIPNPFED   150
FTKDDIDPGA INRAQELRTV YYNMPLPKDM IDEEGNPIMQ YPRNKIRTTK   200
YTPLTFLPKN ILFQFHNFAN VYFLVLIILG AFQIFGVTNP GLSAVPLVVI   250
VIITAIKDAI EDSRRTVLDL EVNNTKTHIL EGVENENVST DNISLWRRFK   300
KANSRLLFKF IQYCKEHLTE EGKKKRMQRK RHELRVQKTV GTSGPRSSLD   350
SIDSYRVSAD YGRPSLDYDN LEQGAGEANI VDRSLPPRTD CKFAKNYWKG   400
VKVGDIVRIH NNDEIPADII LLSTSDTDGA CYVETKNLDG ETNLKVRQSL   450
KCTNTIRTSK DIARTKFWIE SEGPHSNLYT YQGNMKWRNL ADGEIRNEPI   500
TINNVLLRGC TLRNTKWAMG VVMFTGGDTK IMLNSGITPT KKSRISRELN   550
FSVVINFVLL FILCFVSGIA NGVYYDKKGR SRFSYEFGTI AGSAATNGFV   600
SFWVAVILYQ SLVPISLYIS VEIIKTAQAA FIYGDVLLYN AKLDYPCTPK   650
SWNISDDLGQ VEYIFSDKTG TLTQNVMEFK KCTINGVSYG RAYTEALAGL   700
RKRQGIDVET EGRREKAEIA KDRDTMIDEL RALSGNSQFY PEEVTFVSKE   750
FVRDLKGASG EVQQRCCEHF MLALALCHSV LVEANPDNPK KLDLKAQSPD   800
EAALVATARD VGFSFVGKTK KGLIIEMQGI QKEFEILNIL EFNSSRKRMS   850
CIVKIPGLNP GDEPRALLIC KGADSIIYSR LSRQSGSNSE AILEKTALHL   900
EQYATEGLRT LCIAQRELSW SEYEKWNEKY DIAAASLANR EDELEVVADS   950
IERELILLGG TAIEDRLQDG VPDCIELLAE AGIKLWVLTG DKVETAINIG   1000
FSCNLLNNEM ELLVIKTTGD DVKEFGSEPS EIVDALLSKY LKEYFNLTGS   1050
EEEIFEAKKD HEFPKGNYAI VIDGDALKLA LYGEDIRRKF LLLCKNCRAV   1100
LCCRVSPSQK AAVVKLVKDS LDVMTLAIGD GSNDVAMIQS ADVGIGIAGE   1150
EGRQAVMCSD YAIGQFRYLA RLVLVHGRWS YKRLAEMIPE FFYKNMIFAL   1200
ALFWYGIYND FDGSYLYEYT YMMFYNLAFT SLPVIFLGIL DQDVNDTISL   1250
VVPQLYRVGI LRKEWNQRKF LWYMLDGLYQ SIICFFFPYL VYHKNMIVTS   1300
NGLGLDHRYF VGVYVTTIAV ISCNTYVLLH QYRWDWFSGL FIALSCLVVF   1350
AWTGIWSSAI ASREFFKAAA RIYGAPSFWA VFFVAVLFCL LPRFTYDSFQ   1400
KFFYPTDVEI VREMWQHGHF DHYPPGYDPT DPNRPKVTKA GQHGEKIIEG   1450
IALSDNLGGS NYSRDSVVTE EIPMTFMHGE DGSPSGYQKQ ETWMTSPKET   1500
QDLLQSPQFQ QAQTFGRGPS TNVRSSLDRT REQMIATNQL DNRYSVERAR   1550
TSLDLPGVTN AASLIGTQQN N                                  1571