SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDLD3
Systematic nameYEL071W
DescriptionD-lactate dehydrogenase [cytochrome] 3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K17        
2         
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP39976
SGD YEL071W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005625soluble fractionIDA:SGD.
Molecular functionGO:0004458D-lactate dehydrogenase (cytochrome) activityIDA:SGD.
EC number 1.1.2.4

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length496
Molecular weight55225

	---------+----------+----------+----------+----------+
MTAAHPVAQL TAEAYPKVKR NPNFKVLDSE DLAYFRSILS NDEILNSQAP   50
EELASFNQDW MKKYRGQSNL ILLPNSTDKV SKIMKYCNDK KLAVVPQGGN   100
TDLVGASVPV FDEIVLSLRN MNKVRDFDPV SGTFKCDAGV VMRDAHQFLH   150
DHDHIFPLDL PSRNNCQVGG VVSTNAGGLN FLRYGSLHGN VLGLEVVLPN   200
GEIISNINAL RKDNTGYDLK QLFIGAEGTI GVVTGVSIVA AAKPKALNAV   250
FFGIENFDTV QKLFVKAKSE LSEILSAFEF MDRGSIECTI EYLKDLPFPL   300
ENQHNFYVLI ETSGSNKRHD DEKLTAFLKD TTDSKLISEG MMAKDKADFD   350
RLWTWRKSVP TACNSYGGMY KYDMSLQLKD LYSVSAAVTE RLNAAGLIGD   400
APKPVVKSCG YGHVGDGNIH LNIAVREFTK QIEDLLEPFV YEYIASKKGS   450
ISAEHGIGFH KKGKLHYTRS DIEIRFMKDI KNHYDPNGIL NPYKYI       496

	* Ubiquitinated lysine is denoted as red color.