SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDHH1
Systematic nameYDL160C
DescriptionATP-dependent RNA helicase DHH1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP39517
SGD YDL160C
Gene Ontology (GO)
Cellular componentGO:0000932cytoplasmic mRNA processing bodyIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0000290deadenylation-dependent decappingIMP:SGD.
EC number 3.6.1.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length506
Molecular weight57544

	---------+----------+----------+----------+----------+
MGSINNNFNT NNNSNTDLDR DWKTALNIPK KDTRPQTDDV LNTKGNTFED   50
FYLKRELLMG IFEAGFEKPS PIQEEAIPVA ITGRDILARA KNGTGKTAAF   100
VIPTLEKVKP KLNKIQALIM VPTRELALQT SQVVRTLGKH CGISCMVTTG   150
GTNLRDDILR LNETVHILVG TPGRVLDLAS RKVADLSDCS LFIMDEADKM   200
LSRDFKTIIE QILSFLPPTH QSLLFSATFP LTVKEFMVKH LHKPYEINLM   250
EELTLKGITQ YYAFVEERQK LHCLNTLFSK LQINQAIIFC NSTNRVELLA   300
KKITDLGYSC YYSHARMKQQ ERNKVFHEFR QGKVRTLVCS DLLTRGIDIQ   350
AVNVVINFDF PKTAETYLHR IGRSGRFGHL GLAINLINWN DRFNLYKIEQ   400
ELGTEIAAIP ATIDKSLYVA ENDETVPVPF PIEQQSYHQQ AIPQQQLPSQ   450
QQFAIPPQQH HPQFMVPPSH QQQQAYPPPQ MPSQQGYPPQ QEHFMAMPPG   500
QSQPQY                                                   506