SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDFG5
Systematic nameYMR238W
DescriptionMannan endo-1,6-alpha-mannosidase DFG5

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ05031
SGD YMR238W
Gene Ontology (GO)
Cellular componentGO:0046658anchored to plasma membraneIDA:SGD.
Biological processGO:0007117budding cell bud growthIGI:SGD.
Biological processGO:0009272chitin- and beta-glucan-containing cell wal...IDA:SGD.
Biological processGO:0007124pseudohyphal growthIMP:SGD.
EC number 3.2.1.101

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length458
Molecular weight50541

	---------+----------+----------+----------+----------+
MIVNISAKMI LSICFTFLSF FKATHAMDLD TTSKTSICDA TALIQGGMLD   50
YYEGTRYGGT VGMFQSPYYW WHAGEAFGGM LENWFLCEND TYQELLYDAL   100
LAQTGSNYDY IPSNQTMVEG NDDQGIWGIT VMGAVERNFT DPGDGKPGWL   150
AMVQAVFNTM YSRWDSEHCG GGLRWQIFTW NSGYNYKNTV SNACLFQIAA   200
RLGRYTGNTT YLEVAEQVFD WLVDVGYVVL NDTANVFDGA EIDTNCTDIT   250
KIEWTYNHGI VLGGLAYMYN ATNGTGEWET SLTKILNGAK SYFFKDSIMY   300
ESACQDYGTC NTDQRTFKSI FSRMLGLTSV MAPFTRDTID DLIKTSAEAA   350
AKSCNGGTDG HTCGLNWQKQ TNDGYYGLGE QMSALEVIQN LLIHDRPAPY   400
KEDNGGTSKG DANAGMNSST TNVLQNNLNI KKGDRAGAAI ITAVILSVLT   450
GGAVWMLF                                                 458