SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDED81
Systematic nameYHR019C
DescriptionAsparaginyl-tRNA synthetase, cytoplasmic

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP38707
SGD YHR019C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmTAS:SGD.
Molecular functionGO:0004816asparagine-tRNA ligase activityIDA:SGD.
Molecular functionGO:0005524ATP bindingTAS:SGD.
Biological processGO:0006421asparaginyl-tRNA aminoacylationIDA:SGD.
EC number 6.1.1.22

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length554
Molecular weight62207

	---------+----------+----------+----------+----------+
MSSLYIKEAT GVDELTTAGS QDHPFKTPAY ALFASQQKSD ATEPKLFVFK   50
TEDNEYQEIS ASALKKARKG CDGLKKKAVK QKEQELKKQQ KEAENAAKQL   100
SALNITIKED ESLPAAIKTR IYDSYSKVGQ RVKVSGWIHR LRSNKKVIFV   150
VLRDGSGFIQ CVLSGDLALA QQTLDLTLES TVTLYGTIVK LPEGKTAPGG   200
VELNVDYYEV VGLAPGGEDS FTNKIAEGSD PSLLLDQRHL ALRGDALSAV   250
MKVRAALLKS VRRVYDEEHL TEVTPPCMVQ TQVEGGSTLF KMNYYGEEAY   300
LTQSSQLYLE TCLASLGDVY TIQESFRAEK SHTRRHLSEY THIEAELAFL   350
TFDDLLQHIE TLIVKSVQYV LEDPIAGPLV KQLNPNFKAP KAPFMRLQYK   400
DAITWLNEHD IKNEEGEDFK FGDDIAEAAE RKMTDTIGVP IFLTRFPVEI   450
KSFYMKRCSD DPRVTESVDV LMPNVGEITG GSMRIDDMDE LMAGFKREGI   500
DTDAYYWFID QRKYGTCPHG GYGIGTERIL AWLCDRFTVR DCSLYPRFSG   550
RCKP                                                     554