SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDED1
Systematic nameYOR204W
AliasSPP81
DescriptionATP-dependent RNA helicase DED1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP06634
SGD YOR204W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIC:SGD.
Molecular functionGO:0003724RNA helicase activityIDA:SGD.
Biological processGO:0008380RNA splicingIDA:SGD.
Biological processGO:0006413translational initiationIMP:SGD.
EC number 3.6.1.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
535Phosphoserine.
539Phosphoserine.
541Phosphoserine.
543Phosphoserine.
572Phosphoserine.
576Phosphoserine.

Sequence Information
Sequence length604
Molecular weight65553

	---------+----------+----------+----------+----------+
MAELSEQVQN LSINDNNENG YVPPHLRGKP RSARNNSSNY NNNNGGYNGG   50
RGGGSFFSNN RRGGYGNGGF FGGNNGGSRS NGRSGGRWID GKHVPAPRNE   100
KAEIAIFGVP EDPNFQSSGI NFDNYDDIPV DASGKDVPEP ITEFTSPPLD   150
GLLLENIKLA RFTKPTPVQK YSVPIVANGR DLMACAQTGS GKTGGFLFPV   200
LSESFKTGPS PQPESQGSFY QRKAYPTAVI MAPTRELATQ IFDEAKKFTY   250
RSWVKACVVY GGSPIGNQLR EIERGCDLLV ATPGRLNDLL ERGKISLANV   300
KYLVLDEADR MLDMGFEPQI RHIVEDCDMT PVGERQTLMF SATFPADIQH   350
LARDFLSDYI FLSVGRVGST SENITQKVLY VENQDKKSAL LDLLSASTDG   400
LTLIFVETKR MADQLTDFLI MQNFRATAIH GDRTQSERER ALAAFRSGAA   450
TLLVATAVAA RGLDIPNVTH VINYDLPSDV DDYVHRIGRT GRAGNTGLAT   500
AFFNSENSNI VKGLHEILTE ANQEVPSFLK DAMMSAPGSR SNSRRGGFGR   550
NNNRDYRKAG GASAGGWGSS RSRDNSFRGG SGWGSDSKSS GWGNSGGSNN   600
SSWW                                                     604