SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameDBP1
Systematic nameYPL119C
DescriptionATP-dependent RNA helicase DBP1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP24784
SGD YPL119C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Biological processGO:0006397mRNA processingIGI:SGD.
Biological processGO:0006413translational initiationIMP:SGD.
EC number 3.6.1.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length617
Molecular weight67917

	---------+----------+----------+----------+----------+
MADLPQKVSN LSINNKENGG GGGKSSYVPP HLRSRGKPSF ERSTPKQEDK   50
VTGGDFFRRA GRQTGNNGGF FGFSKERNGG TSANYNRGGS SNYKSSGNRW   100
VNGKHIPGPK NAKLEAELFG VHDDPDYHSS GIKFDNYDNI PVDASGKDVP   150
EPILDFSSPP LDELLMENIK LASFTKPTPV QKYSIPIVTK GRDLMACAQT   200
GSGKTGGFLF PLFTELFRSG PSPVPEKAQS FYSRKGYPSA LVLAPTRELA   250
TQIFEEARKF TYRSWVRPCV VYGGAPIGNQ MREVDRGCDL LVATPGRLND   300
LLERGKVSLA NIKYLVLDEA DRMLDMGFEP QIRHIVEECD MPSVENRQTL   350
MFSATFPVDI QHLARDFLDN YIFLSVGRVG STSENITQRI LYVDDMDKKS   400
ALLDLLSAEH KGLTLIFVET KRMADQLTDF LIMQNFKATA IHGDRTQAER   450
ERALSAFKAN VADILVATAV AARGLDIPNV THVINYDLPS DIDDYVHRIG   500
RTGRAGNTGV ATSFFNSNNQ NIVKGLMEIL NEANQEVPTF LSDLSRQNSR   550
GGRTRGGGGF FNSRNNGSRD YRKHGGNGSF GSTRPRNTGT SNWGSIGGGF   600
RNDNEKNGYG NSNASWW                                       617