SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCYS4
Systematic nameYGR155W
AliasSTR4
DescriptionCystathionine beta-synthase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K235        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32582
SGD YGR155W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004122cystathionine beta-synthase activityIDA:SGD.
Biological processGO:0019344cysteine biosynthetic processIMP:SGD.
Biological processGO:0042493response to drugIMP:SGD.
EC number 4.2.1.22

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
350Phosphoserine.

Sequence Information
Sequence length507
Molecular weight56022

	---------+----------+----------+----------+----------+
MTKSEQQADS RHNVIDLVGN TPLIALKKLP KALGIKPQIY AKLELYNPGG   50
SIKDRIAKSM VEEAEASGRI HPSRSTLIEP TSGNTGIGLA LIGAIKGYRT   100
IITLPEKMSN EKVSVLKALG AEIIRTPTAA AWDSPESHIG VAKKLEKEIP   150
GAVILDQYNN MMNPEAHYFG TGREIQRQLE DLNLFDNLRA VVAGAGTGGT   200
ISGISKYLKE QNDKIQIVGA DPFGSILAQP ENLNKTDITD YKVEGIGYDF   250
VPQVLDRKLI DVWYKTDDKP SFKYARQLIS NEGVLVGGSS GSAFTAVVKY   300
CEDHPELTED DVIVAIFPDS IRSYLTKFVD DEWLKKNNLW DDDVLARFDS   350
SKLEASTTKY ADVFGNATVK DLHLKPVVSV KETAKVTDVI KILKDNGFDQ   400
LPVLTEDGKL SGLVTLSELL RKLSINNSNN DNTIKGKYLD FKKLNNFNDV   450
SSYNENKSGK KKFIKFDENS KLSDLNRFFE KNSSAVITDG LKPIHIVTKM   500
DLLSYLA                                                  507

	* Ubiquitinated lysine is denoted as red color.