SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCYM1
Systematic nameYDR430C
AliasMOP112
DescriptionMitochondrial presequence protease

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32898
SGD YDR430C
Gene Ontology (GO)
Cellular componentGO:0005758mitochondrial intermembrane spaceIDA:SGD.
Molecular functionGO:0004222metalloendopeptidase activityIDA:SGD.
Biological processGO:0006508proteolysisIDA:SGD.
EC number 3.4.24.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
920Phosphoserine.

Sequence Information
Sequence length989
Molecular weight112180

	---------+----------+----------+----------+----------+
MLRFQRFASS YAQAQAVRKY PVGGIFHGYE VRRILPVPEL RLTAVDLVHS   50
QTGAEHLHID RDDKNNVFSI AFKTNPPDST GVPHILEHTT LCGSVKYPVR   100
DPFFKMLNKS LANFMNAMTG PDYTFFPFST TNPQDFANLR GVYLDSTLNP   150
LLKQEDFDQE GWRLEHKNIT DPESNIVFKG VVYNEMKGQI SNANYYFWSK   200
FQQSIYPSLN NSGGDPMKIT DLRYGDLLDF HHKNYHPSNA KTFTYGNLPL   250
VDTLKQLNEQ FSGYGKRARK DKLLMPIDLK KDIDVKLLGQ IDTMLPPEKQ   300
TKASMTWICG APQDTYDTFL LKVLGNLLMD GHSSVMYQKL IESGIGLEFS   350
VNSGVEPTTA VNLLTVGIQG VSDIEIFKDT VNNIFQNLLE TEHPFDRKRI   400
DAIIEQLELS KKDQKADFGL QLLYSILPGW TNKIDPFESL LFEDVLQRFR   450
GDLETKGDTL FQDLIRKYIV HKPCFTFSIQ GSEEFSKSLD DEEQTRLREK   500
ITALDEQDKK NIFKRGILLQ EKQNEKEDLS CLPTLQIKDI PRAGDKYSIE   550
QKNNTMSRIT DTNGITYVRG KRLLNDIIPF ELFPYLPLFA ESLTNLGTTT   600
ESFSEIEDQI KLHTGGISTH VEVTSDPNTT EPRLIFGFDG WSLNSKTDHI   650
FEFWSKILLE TDFHKNSDKL KVLIRLLASS NTSSVADAGH AFARGYSAAH   700
YRSSGAINET LNGIEQLQFI NRLHSLLDNE ETFQREVVDK LTELQKYIVD   750
TNNMNFFITS DSDVQAKTVE SQISKFMERL PHGSCLPNGP KTSDYPLIGS   800
KCKHTLIKFP FQVHYTSQAL LGVPYTHKDG SALQVMSNML TFKHLHREVR   850
EKGGAYGGGA SYSALAGIFS FYSYRDPQPL KSLETFKNSG RYILNDAKWG   900
VTDLDEAKLT IFQQVDAPKS PKGEGVTYFM SGVTDDMKQA RREQLLDVSL   950
LDVHRVAEKY LLNKEGVSTV IGPGIEGKTV SPNWEVKEL               989