SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCYC1
Systematic nameYJR048W
DescriptionCytochrome c iso-1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K10MULTI(K48-linked)       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
Methodautoradiography
Reference"Ubiquitin conjugation to cytochromes c. Structure of the yeast iso-1 conjugate and possible recognition determinants."
Sokolik CW, Cohen RE.
J Biol Chem. 267(2):1067-71 (1992) [1309759]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP00044
SGD YJR048W
Gene Ontology (GO)
Cellular componentGO:0005758mitochondrial intermembrane spaceIDA:SGD.
Molecular functionGO:0009055electron carrier activityTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006118electron transportTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
78N6,N6,N6-trimethyllysine, by CTM1.

Sequence Information
Sequence length109
Molecular weight12182

	---------+----------+----------+----------+----------+
MTEFKAGSAK KGATLFKTRC LQCHTVEKGG PHKVGPNLHG IFGRHSGQAE   50
GYSYTDANIK KNVLWDENNM SEYLTNPKKY IPGTKMAFGG LKKEKDRNDL   100
ITYLKKACE                                                109

	* Ubiquitinated lysine is denoted as red color.