SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCUL3
Systematic nameYGR003W
DescriptionCullin-3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53202
SGD YGR003W
Gene Ontology (GO)
Cellular componentGO:0031463Cul3-RING ubiquitin ligase complexIDA:SGD.
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0042787protein ubiquitination during ubiquitin-dep...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length744
Molecular weight86115

	---------+----------+----------+----------+----------+
MITNKKIKIS VPEKLGLSEE SFEESWETVK YAIDHIYSDD MADLSFEQVY   50
KTIYTIVLNK KGPILYNRLK DYLIQKLALL RETIVKDNTH DYEFLGTMAR   100
LWEVQCHCFK ITGDLMMYMD KVYCKPNRCM EVYDMCLDLF RIEILQKCSS   150
SLISALISDI ERIRNLGSVD SEHTSLWKVL IGMMETLHDN RDSFFLTDFE   200
PVLISATEEY YNKAIDIELL TPIESLEKIR KLRQFESMLD SSFLNVDSHN   250
KLKTVLENVL IWGKLSDIIE DLTHEAMVIS NGKLLQEIYD LSSEEKYRVT   300
VIESIKSYIN KNAINIPFNE GNRKKGQNAI TWSSEIVELY RSQHSFLESI   350
DFGSVRLNNL TGDVSNAILG DVFSMYFSKE GALPSEYLST YVDHCMKRTK   400
EKDAEIVKIK QDLLDSTKLI GLLTEKDIFE KIYKKQLSRR LLQQRSLVEI   450
EKWMVQMIKK VLGTFFTSKL EIMLRDISLS SEMYQAFQHS TINSIEYLSF   500
APQVLTRTSW PFQSTNPIDE GISLPPRMSQ ILAGFEGYYS LKYKERVLKW   550
AHHLSVIEIG CQFNSGYYEI SFSVYAGVIF LLFEDYEELT LGEIYELTHI   600
PIDDVKSLVM SMSTIPRCKI LKKSSSSGNM KFSVNYFFSS PNRKVKVPVI   650
ACPLPSQKSD NLATASSVDT YDNEIVMELS AIIVRIMKTE GKLSHQQLLE   700
RTTKRTQSRL SLTPSILKRS IQLLIEKEYI QRNADDPSYY HYLS         744