SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCUE5
Systematic nameYOR042W
DescriptionUbiquitin-binding protein CUE5

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K76;K396        
2   HECTNARSP5   
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ08412
SGD YOR042W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
11Phosphoserine.
36Phosphoserine.
50Phosphothreonine.
70Phosphothreonine.
91Phosphoserine.
167Phosphothreonine.
220Phosphoserine.
348Phosphoserine.
351Phosphoserine.
352Phosphothreonine.
364Phosphothreonine.
367Phosphothreonine.
384Phosphothreonine.
407Phosphoserine.

Sequence Information
Sequence length411
Molecular weight46870

	---------+----------+----------+----------+----------+
MEEKEGIKDS SLLEKSNVPE SINEDISKTT DVDLNSDGKK DNDTSAKDGT   50
PKVEEKVNKS SGIDEDEVVT PAEDAKEEEE EHPPLPARRK SEEEPSKENP   100
ILQELKDAFP NLEEKYIKAV IIASQGVLSP AFNALLFLSD PESGKDIELP   150
TQPVRKNPEA PARRRQTQLE QDELLARQLD EQFNSSHSRR RNRDRATRSM   200
HEQRRRRHNP NEREQHHEDS EEEDSWSQFV EKDLPELTDR AGRSLQDTAN   250
KVSNWISDAY RRNFASGNEQ NDNQHGHQDQ QEWEPEIVDL SQGGKNSRPQ   300
QPERRRFNSF GVQVGDDSLE SHGITLHNED GFEDDEDVPP QLPTRTKSGE   350
STGKVVAETT YIDTPDTETK KKWQPLPPEP LDTTPTKVNA VSRNKKNPDE   400
DEFLINSDDE M                                             411

	* Ubiquitinated lysine is denoted as red color.