SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCTR9
Systematic nameYOL145C
AliasCDP1
DescriptionRNA polymerase-associated protein CTR9

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K196        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP89105
SGD YOL145C
Gene Ontology (GO)
Cellular componentGO:0016593Cdc73/Paf1 complexIPI:SGD.
Molecular functionGO:0016944RNA polymerase II transcription elongation ...IPI:SGD.
Molecular functionGO:0045142triplex DNA bindingIDA:SGD.
Biological processGO:0007059chromosome segregationIMP:SGD.
Biological processGO:0016571histone methylationIMP:SGD.
Biological processGO:0006368RNA elongation from RNA polymerase II promoterIPI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
966Phosphoserine.
974Phosphoserine.
977Phosphoserine.
1015Phosphoserine.
1017Phosphoserine.

Sequence Information
Sequence length1077
Molecular weight124655

	---------+----------+----------+----------+----------+
MTNAMKVEGY PSMEWPTSLD IPLKASEELV GIDLETDLPD DPTDLKTLLV   50
EENSEKEHWL TIALAYCNHG KTNEGIKLIE MALDVFQNSE RASLHTFLTW   100
AHLNLAKGQS LSVETKEHEL TQAELNLKDA IGFDPTWIGN MLATVELYYQ   150
RGHYDKALET SDLFVKSIHA EDHRSGRQSK PNCLFLLLRA KLLYQKENYM   200
ASLKIFQELL VINPVLQPDP RIGIGLCFWQ LKDSKMAIKS WQRALQLNPK   250
NTSASILVLL GEFRESFTNS TNDKTFKEAF TKALSDLNNI FSENQHNPVL   300
LTLLQTYYYF KGDYQTVLDI YHHRILKMSP MIAKIVLSES SFWCGRAHYA   350
LGDYRKSFIM FQESLKKNED NLLAKLGLGQ TQIKNNLLEE SIITFENLYK   400
TNESLQELNY ILGMLYAGKA FDAKTAKNTS AKEQSNLNEK ALKYLERYLK   450
LTLATKNQLV ISRAYLVISQ LYELQNQYKT SLDYLSKALE EMEFIKKEIP   500
LEVLNNLACY HFINGDFIKA DDLFKQAKAK VSDKDESVNI TLEYNIARTN   550
EKNDCEKSES IYSQVTSLHP AYIAARIRNL YLKFAQSKIE DSDMSTEMNK   600
LLDLNKSDLE IRSFYGWYLK NSKERKNNEK STTHNKETLV KYNSHDAYAL   650
ISLANLYVTI ARDGKKSRNP KEQGKSKHSY LKAIQLYQKV LQVDPFNIFA   700
AQGLAIIFAE SKRLGPALEI LRKVRDSLDN EDVQLNLAHC YLEMREYGKA   750
IENYELVLKK FDNEKTRPHI LNLLGRAWYA RAIKETSVNF YQKALENAKT   800
ALDLFVKESS KSKFIHSVKF NIALLHFQIA ETLRRSNPKF RTVQQIKDSL   850
EGLKEGLELF RELNDLKEFN MIPKEELEQR IQLGETTMKS ALERSLNEQE   900
EFKKEQRAKI DEARKILEEN ELKEQGWMKQ EEEARRLKLE KQAEEYRKLQ   950
DEAQKIFQVR EAMAISEHNV KDDSDLSDKD NEYDEEQPRQ KRKRSTKTKN   1000
SGESKRRKAA KKTLSDSDED DDDVVKKPSH NKGKKSQLSN EFIEDSDEEE   1050
AQMSGSEQNK NDDNDENNDN DDNDGLF                            1077

	* Ubiquitinated lysine is denoted as red color.