SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameCTF13
Systematic nameYMR094W
AliasCBF3C
DescriptionCentromere DNA-binding protein complex CBF3 subunit C

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGSCFCDC4   
2 AUTOCDC34RINGSCFCTF13   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Regulating the yeast kinetochore by ubiquitin-dependent degradation and Skp1p-mediated phosphorylation."
Kaplan KB, Hyman AA, Sorger PK.
Cell. 91(4):491-500 (1997) [9390558]
[2]EvidenceHigh MW Product Confirmation
MethodWestern blot (in vitro)
Reference"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
Kus BM, Caldon CE, Andorn-Broza R, Edwards AM.
Proteins. 54(3):455-67 (2004) [14747994]

Cross References
Swiss-Prot accessionP35203
SGD YMR094W
Gene Ontology (GO)
Cellular componentGO:0031518CBF3 complexIDA:SGD.
Cellular componentGO:0000778condensed nuclear chromosome kinetochoreTAS:SGD.
Molecular functionGO:0019237centromeric DNA bindingTAS:SGD.
Molecular functionGO:0008301DNA bending activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0051382kinetochore assemblyIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length478
Molecular weight56336

	---------+----------+----------+----------+----------+
MPSFNPVRFL ELPIDIRKEV YFHLDGNFCG AHPYPIDILY KSNDVELPGK   50
PSYKRSKRSK KLLRYMYPVF ATYLNIFEYS PQLIEKWLEY AFWLRYDCLV   100
LDCFKVNHLY DGTLIDALEW TYLDNELRLA YFNKASMLEV WYTFKEYKKW   150
VIDSVAFDEL DLLNVSNIQF NIDNLTPQLV DKCLSILEQK DLFATIGEVQ   200
FGQDEEVGEE KDVDVSGANS DENSSPSSTI KNKKRSASKR SHSDNGNVGA   250
THNQLTSISV IRTIRSMESM KSLRKITVRG EKLYELLINF HGFRDNPGKT   300
ISYIVKRRIN EIRLSRMNQI SRTGLADFTR WDNLQKLVLS RVAYIDLNSI   350
VFPKNFKSLT MKRVSKIKWW NIEENILKEL KVDKRTFKSL YIKEDDSKFT   400
KFFNLRHTRI KELDKSEINQ ITYLRCQAIV WLSFRTLNHI KLQNVSEVFN   450
NIIVPRALFD SKRVEIYRCE KISQVLVI                           478